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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Keratin 12/K12 antibody (ab169328)

  • Datasheet
  • SDS
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Western blot - Anti-Keratin 12/K12 antibody (ab169328)

    Key features and details

    • Mouse polyclonal to Keratin 12/K12
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Keratin 12/K12 antibody
      See all Keratin 12/K12 primary antibodies
    • Description

      Mouse polyclonal to Keratin 12/K12
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Dog
    • Immunogen

      Full length protein corresponding to Human Keratin 12/K12 aa 1-494. (AAI56642.1)
      Sequence:

      MDLSNNTMSLSVRTPGLSRRLSSQSVIGRPRGMSASSVGSGYGGSAFGFG ASCGGGFSAASMFGSSSGFGGGSGSSMAGGLGAGYGRALGGGSFGGLGMG FGGSPGGGSLGILSGNDGGLLSGSEKETMQNLNDRLASYLDKVRALEEAN TELENKIREWYETRGTGTADASQSDYSKYYPLIEDLRNKIISASIGNAQL LLQIDNARLAAEDFRMKYENELALRQGVEADINGLRRVLDELTLTRTDLE MQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLND MRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRR AFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQ VRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTD SKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM


      Database link: Q99456
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Keratin 12/K12-transfected 293T cell lysate.
    • General notes

      Previously labelled as Keratin 12. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.4
      Constituent: 100% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class I
      • Cytokeratins
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types
      • Neuroscience
      • Sensory System
      • Visual system

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab169328 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.

    Target

    • Function

      May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.
    • Tissue specificity

      Cornea specific.
    • Involvement in disease

      Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Target information above from: UniProt accession Q99456 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3859 Human
      • Omim: 601687 Human
      • SwissProt: Q99456 Human
      • Unigene: 66739 Human
      • Alternative names

        • CK-12 antibody
        • Cytokeratin-12 antibody
        • K12 antibody
        • K1C12_HUMAN antibody
        • Keratin 12 antibody
        • Keratin antibody
        • Keratin, type I cytoskeletal 12 antibody
        • Keratin-12 antibody
        • KRT12 antibody
        • type I cytoskeletal 12 antibody
        see all

      Images

      • Western blot - Anti-Keratin 12/K12 antibody (ab169328)
        Western blot - Anti-Keratin 12/K12 antibody (ab169328)
        All lanes : Anti-Keratin 12/K12 antibody (ab169328) at 1 µg/ml

        Lane 1 : Keratin 12/K12-transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 15 µl per lane.

        Developed using the ECL technique.

        Predicted band size: 55 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab169328? Please let us know so that we can cite the reference in this datasheet.

      ab169328 has been referenced in 1 publication.

      • Sun N  et al. Expression and influence of BMP-4 in human dental pulp cells cultured in vitro. Exp Ther Med 16:5112-5116 (2018). PubMed: 30542466

      Customer reviews and Q&As

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