Recombinant Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1609(2)] to Keratin 12/K12
- Suitable for: WB
- Reacts with: Rat
Overview
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Product name
Anti-Keratin 12/K12 antibody [EPR1609(2)]
See all Keratin 12/K12 primary antibodies -
Description
Rabbit monoclonal [EPR1609(2)] to Keratin 12/K12 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Rat -
Immunogen
corresponding to Human Keratin 12/K12 aa 450-550 (C terminal).
Database link: Q99456 -
Positive control
- Rat eyeball lysate.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as Keratin 12.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1609(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Rat
|
All applications |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa. |
Target
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Function
May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. -
Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3859 Human
- Entrez Gene: 360625 Rat
- Omim: 601687 Human
- SwissProt: Q99456 Human
- SwissProt: Q6IFW5 Rat
- Unigene: 66739 Human
- Unigene: 94852 Rat
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Alternative names
- CK-12 antibody
- Cytokeratin-12 antibody
- K12 antibody
see all
Images
Datasheets and documents
References (3)
ab124975 has been referenced in 3 publications.
- Guo D et al. A Spatiotemporal Requirement for Prickle 1-Mediated PCP Signaling in Eyelid Morphogenesis and Homeostasis. Invest Ophthalmol Vis Sci 59:952-966 (2018). PubMed: 29450535
- Li G et al. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. J Biol Chem 290:20448-54 (2015). PubMed: 26045558
- Ouyang H et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature 511:358-61 (2014). PubMed: 25030175