Product nameAnti-Keratin 12/K12 antibody [EPR1609(2)]
See all Keratin 12/K12 primary antibodies
DescriptionRabbit monoclonal [EPR1609(2)] to Keratin 12/K12
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Rat, Human
corresponding to Human Keratin 12/K12 aa 450-550 (C terminal).
Database link: Q99456
- Rat eyeball lysate.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as Keratin 12.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
FunctionMay play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.
Tissue specificityCornea specific.
Involvement in diseaseDefects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK-12 antibody
- Cytokeratin-12 antibody
- K12 antibody
This product has been referenced in:
- Li G et al. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. J Biol Chem 290:20448-54 (2015). Read more (PubMed: 26045558) »
- Ouyang H et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature 511:358-61 (2014). Read more (PubMed: 25030175) »