Recombinant
RabMAb

Recombinant Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)

Submit a review Q&A (1)References (2)

Overview

  • Product name

    Anti-Keratin 12/K12 antibody [EPR1609(2)]
    See all Keratin 12/K12 primary antibodies

  • Description

    Rabbit monoclonal [EPR1609(2)] to Keratin 12/K12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    corresponding to Human Keratin 12/K12 aa 450-550 (C terminal).
    Database link: Q99456

  • Positive control

    • Rat eyeball lysate.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Previously labelled as Keratin 12. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab124975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.

    • Tissue specificity

      Cornea specific.

    • Involvement in disease

      Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.

    • Sequence similarities

      Belongs to the intermediate filament family.
    • Information by UniProt

    • Database links

      • Alternative names

        • CK-12 antibody
        • Cytokeratin-12 antibody
        • K12 antibody
        • K1C12_HUMAN antibody
        • Keratin 12 antibody
        • Keratin antibody
        • Keratin, type I cytoskeletal 12 antibody
        • Keratin-12 antibody
        • KRT12 antibody
        • type I cytoskeletal 12 antibody
        see all

      Images

      • Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
        Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
        Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975) at 1/1000 dilution + Rat eyeball lysate at 10 µg

        Predicted band size: 54 kDa

      References

      This product has been referenced in:

      • Li G  et al. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. J Biol Chem 290:20448-54 (2015). Read more (PubMed: 26045558) »
      • Ouyang H  et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature 511:358-61 (2014). Read more (PubMed: 25030175) »
      See all 2 Publications for this product

      Publishing research using ab124975? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I need an antibody that is tagged with FITC or APC or some fluorescence so that I can use them in flow cytometry. Im looking for K3 and K12. It would be awesome if you can also provide me with the same information about p63 and ABCG2. Thank you for your time.

      Read More
      Answer

      Thank you for your inquiry and your interest in our products.

      I searched our catalogue and we currently do not have a cytokeratin 3 antibody that is directly conjugated. We do carry ab77869, which has been tested in FC with rabbit, cow and human which could be conjugated to FITC using one of our EasyLink conjugation kits, like ab102885.

      https://www.abcam.com/cytokeratin-3-antibody-ae5-ab77869.html

      https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

      For cytokeratin 12, we do not carry any antibodies that have been tested in flow cytometry. However, if you would like to test ab124975 or ab113039 in FC, we could offer you a discount on a future purchase if you purchase, test, and submit an Abreview on the product (the discount is worth the price you pay for the antibody).

      For p63, we have an antibody conjugated to DyLight 488 that works in ICC/IF with human cells, ab139929. Again, if you would like to test this antibody in FC, we could offer you a future discount. https://www.abcam.com/p63-antibody-epr5701-dylight-488-ab139929.html

      For BCRP/ABCG2, we have 4 antibodies tested in FC, but non are conjugated to a fluorophore: ab24115, ab24114, and ab72788, and ab95692 (conjugated to biotin). You could use the purified antibody ab72788 with one of our conjugation kits to directly conjugate a fluorophore to the antibody.

      I hope this information helps. If you are interested in testing any of the antibodies as listed above, please let me know and I will set you up in our system.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com