Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17882] to Keratin 12/K12 (Alexa Fluor® 488)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Product nameAnti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 488)
See all Keratin 12/K12 primary antibodies
DescriptionRabbit monoclonal [EPR17882] to Keratin 12/K12 (Alexa Fluor® 488)
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human Keratin 12/K12 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q99456
- ICC/IF: Hek293T cells transfected with RFP-tagged Keratin 12/K12.
Previously labelled as Keratin 12.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 594) (ab219144)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 647) (ab222915)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 555) (ab222916)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 568) (ab222917)
- Anti-Keratin 12/K12 antibody [EPR17882] - BSA and Azide free (ab240295)
Our Abpromise guarantee covers the use of ab222116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100. This product gave a positive signal in Hek293T cells transfected with RFP-tagged Keratin 12 fixed with 4% formaldehyde (10 min)|
FunctionMay play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.
Tissue specificityCornea specific.
Involvement in diseaseDefects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK-12 antibody
- Cytokeratin-12 antibody
- K12 antibody
ab222116 staining Keratin 12/K12 in Hek293T cells transfected with RFP-tagged Keratin 12/K12 expression construct or RFP-only-tagged expression construct. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with ab222116 at 1/100 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (pseudocolored in white). Nuclear DNA was labelled with DAPI (shown in blue) and RFP is shown in red.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab222116 has not yet been referenced specifically in any publications.