Product nameAnti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 594)
See all Keratin 12/K12 primary antibodies
DescriptionRabbit monoclonal [EPR17882] to Keratin 12/K12 (Alexa Fluor® 594)
ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human Keratin 12/K12 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q99456
- ICC/IF: Hek293T cells transfected with GFP-tagged Keratin 12/K12
Previously labelled as Keratin 12.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 488) (ab222116)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 647) (ab222915)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 555) (ab222916)
- Anti-Keratin 12/K12 antibody [EPR17882] (Alexa Fluor® 568) (ab222917)
- Anti-Keratin 12/K12 antibody [EPR17882] - BSA and Azide free (ab240295)
Our Abpromise guarantee covers the use of ab219144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in Hek293T cells transfected with GFP-tagged Keratin 12 / K12 fixed with 4% formaldehyde (10 min)
FunctionMay play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.
Tissue specificityCornea specific.
Involvement in diseaseDefects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK-12 antibody
- Cytokeratin-12 antibody
- K12 antibody
ab219144 staining Keratin 12/K12 in Hek293T cells transfected with GFP-tagged Keratin 12/K12 expression construct or GFP-only-tagged expression construct. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with ab219144 at 1/100 dilution (pseudocolored in red) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (pseudocolored in white). Nuclear DNA was labelled with DAPI (shown in blue) and GFP is shown in green.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab219144 has not yet been referenced specifically in any publications.