Anti-ketohexokinase antibody (ab154405)
Key features and details
- Rabbit polyclonal to ketohexokinase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ketohexokinase antibody
See all ketohexokinase primary antibodies -
Description
Rabbit polyclonal to ketohexokinase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
A recombinant protein fragment corresponding to a region within amino acids 1-248 of Human ketohexokinase (UniProt ID: P50053).
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Positive control
- HeLa and HepG2 whole cell lysates; mouse liver tissue lysate; A549 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used. |
Target
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Tissue specificity
Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye. -
Pathway
Carbohydrate metabolism; fructose metabolism. -
Involvement in disease
Defects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism. -
Sequence similarities
Belongs to the carbohydrate kinase pfkB family. - Information by UniProt
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Database links
- Entrez Gene: 3795 Human
- Entrez Gene: 16548 Mouse
- Entrez Gene: 25659 Rat
- Omim: 614058 Human
- SwissProt: P50053 Human
- SwissProt: P97328 Mouse
- SwissProt: Q02974 Rat
- Unigene: 567297 Human
see all -
Alternative names
- EC 2.7.1.3 antibody
- Fructokinase antibody
- Hepatic fructokinase antibody
see all
Images
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Anti-ketohexokinase antibody (ab154405) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Anti-ketohexokinase antibody (ab154405) at 1/10000 dilution + mouse liver tissue lysate at 20 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ketohexokinase using ab154405 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154405 has been referenced in 2 publications.
- Jeong S et al. High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells. Cell Metab 33:145-159.e6 (2021). PubMed: 33357456
- Miller CO et al. Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects. Am J Physiol Endocrinol Metab 315:E386-E393 (2018). PubMed: 29870677