Overview

  • Product name

    Anti-ketohexokinase antibody
    See all ketohexokinase primary antibodies
  • Description

    Rabbit polyclonal to ketohexokinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human ketohexokinase aa 1-298.
    Database link: P50053

  • Positive control

    • IHC-P: Human colon cancer and liver tissues. IP: Mouse liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab236768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
IP 1/200 - 1/2000.

Target

  • Tissue specificity

    Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • Pathway

    Carbohydrate metabolism; fructose metabolism.
  • Involvement in disease

    Defects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism.
  • Sequence similarities

    Belongs to the carbohydrate kinase pfkB family.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.7.1.3 antibody
    • Fructokinase antibody
    • Hepatic fructokinase antibody
    • Ketohexokinase antibody
    • Ketohexokinase isoform a antibody
    • KHK antibody
    • KHK_HUMAN antibody
    see all

Images

  • Ketohexokinase was immunoprecipitated from mouse liver tissue lysate using ab236768 at 1/200 dilution.

    Lane 1: Rabbit control IgG in mouse liver tissue lysate.

    Lane 2: ab236768 in mouse liver tissue lysate.

    Lane 3: Mouse liver tissue lysate (10 µg input).

    For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

  • Paraffin-embedded human liver tissue stained for ketohexokinase with ab236768 at a 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human colon cancer tissue stained for ketohexokinase with ab236768 at 1/100 dilution in immunohistochemical analysis.

References

ab236768 has not yet been referenced specifically in any publications.

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