Key features and details
- Rabbit polyclonal to ketohexokinase
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ketohexokinase antibody
See all ketohexokinase primary antibodies
DescriptionRabbit polyclonal to ketohexokinase
Tested applicationsSuitable for: IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human ketohexokinase aa 1-298.
Database link: P50053
- IHC-P: Human colon cancer and liver tissues. IP: Mouse liver tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab236768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|IP||1/200 - 1/2000.|
Tissue specificityMost abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
PathwayCarbohydrate metabolism; fructose metabolism.
Involvement in diseaseDefects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism.
Sequence similaritiesBelongs to the carbohydrate kinase pfkB family.
- Information by UniProt
- EC 188.8.131.52 antibody
- Fructokinase antibody
- Hepatic fructokinase antibody
Ketohexokinase was immunoprecipitated from mouse liver tissue lysate using ab236768 at 1/200 dilution.
Lane 1: Rabbit control IgG in mouse liver tissue lysate.
Lane 2: ab236768 in mouse liver tissue lysate.
Lane 3: Mouse liver tissue lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Paraffin-embedded human liver tissue stained for ketohexokinase with ab236768 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for ketohexokinase with ab236768 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236768 has not yet been referenced specifically in any publications.