Product nameKetone Body Assay Kit
Sample typeUrine, Serum, Plasma, Other biological fluids
Range0.12 mM - 8 mM
Ketone Body Assay Kit (ab272541) is a simple, direct and automation-ready procedure for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH). This ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations.
Sensitive and accurate: Uses 10 μL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.
Convenient: The procedure involves adding a single working reagent, and reading the optical density at room temperature.
High-throughput: Can be automated as a high-throughput 96-well plate assay for many samples per day.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests AcAc Buffer 1 x 20ml AcAc Reagent 1 vial AcAc Standard 1 x 200µl BOH Buffer 1 x 20ml BOH Reagent 1 x 1ml BOH Standard 1 x 200µl HBDH Enzyme 1 x 120µl
Our Abpromise guarantee covers the use of ab272541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272541 has not yet been referenced specifically in any publications.