Anti-KEX2 protease antibody [3B5] (ab34772)
Key features and details
- Mouse monoclonal [3B5] to KEX2 protease
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-KEX2 protease antibody [3B5] -
Description
Mouse monoclonal [3B5] to KEX2 protease -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Saccharomyces cerevisiae KEX2 protease.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
3B5 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 90 kDa. |
Target
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Relevance
Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime. -
Cellular localization
Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 855483 Saccharomyces cerevisiae
- SwissProt: P13134 Saccharomyces cerevisiae
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Alternative names
- KEX 2 antibody
- Kex2p antibody
- Kexin KEX2 antibody
see all
Images
References (2)
ab34772 has been referenced in 2 publications.
- Smindak RJ et al. Lipid-dependent regulation of exocytosis in S. cerevisiae by OSBP homolog (Osh) 4. J Cell Sci 130:3891-3906 (2017). PubMed: 28993464
- Liu Y et al. Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function. Mol Biol Cell 22:1575-84 (2011). WB ; Saccharomyces cerevisiae . PubMed: 21389113