Overview

  • Product name

    Anti-KGF antibody [EPR7261]
    See all KGF primary antibodies
  • Description

    Rabbit monoclonal [EPR7261] to KGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KGF aa 1-100. The exact sequence is proprietary.
    Database link: P21781

  • Positive control

    • WB: Fetal brain and fetal kidney lysates; 293T cell lysate. Human, mouse and rat brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
  • Tissue specificity

    Epithelial cell.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    Potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.
  • Alternative names

    • FGF 7 antibody
    • FGF-7 antibody
    • Fgf7 antibody
    • FGF7_HUMAN antibody
    • Fibroblast growth factor 7 antibody
    • HBGF 7 antibody
    • HBGF-7 antibody
    • HBGF7 antibody
    • Heparin binding growth factor 7 antibody
    • Heparin-binding growth factor 7 antibody
    • Keratinocyte growth factor antibody
    • KGF antibody
    see all

Images

  • All lanes : Anti-KGF antibody [EPR7261] (ab131162) at 0.1 µg/ml (purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-KGF antibody [EPR7261] (ab131162) at 0.1 µg/ml (purified) + Human brain lysates at 15 µg

    Secondary
    Rabbit monoclonal [EPR7261] to KGF (ab131162) at 1/2000 dilution (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)

    Predicted band size: 23 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-KGF antibody [EPR7261] (ab131162) at 1/1000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

References

ab131162 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131162.
Please use the links above to contact us or submit feedback about this product.

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