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    khsrp-antibody-ab229660.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-KHSRP antibody (ab229660)

  • Datasheet
  • SDS
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Western blot - Anti-KHSRP antibody (ab229660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)
  • Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody (ab229660)
  • Western blot - Anti-KHSRP antibody (ab229660)

Key features and details

  • Rabbit polyclonal to KHSRP
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-KHSRP antibody
    See all KHSRP primary antibodies
  • Description

    Rabbit polyclonal to KHSRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Recombinant fragment within Human KHSRP (internal sequence). The exact sequence is proprietary.
    Database link: Q92945

  • Positive control

    • WB: A431 and HeLa whole cell extracts; Mouse brain extract. ICC/IF: SK-N-AS cells. IHC/P: Mouse intestine tissue; Rat duodenum.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab30132)
    • Mouse brain tissue lysate - total protein (ab4022)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • A431 whole cell lysate (ab7909)

Applications

Our Abpromise guarantee covers the use of ab229660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
  • Tissue specificity

    Detected in neural and non-neural cell lines.
  • Sequence similarities

    Belongs to the KHSRP family.
    Contains 4 KH domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
  • Target information above from: UniProt accession Q92945 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374140 Chicken
    • Entrez Gene: 8570 Human
    • Entrez Gene: 16549 Mouse
    • Entrez Gene: 171137 Rat
    • Omim: 603445 Human
    • SwissProt: Q92945 Human
    • SwissProt: Q3U0V1 Mouse
    • SwissProt: Q99PF5 Rat
    • Unigene: 726172 Human
    • Unigene: 34296 Mouse
    • Unigene: 92643 Rat
    see all
  • Alternative names

    • Far upstream element-binding protein 2 antibody
    • FBP2 antibody
    • FUBP2 antibody
    • FUBP2_HUMAN antibody
    • FUSE binding protein 2 antibody
    • FUSE-binding protein 2 antibody
    • KH type splicing regulatory protein (FUSE binding protein 2) antibody
    • KH type splicing regulatory protein antibody
    • KH type-splicing regulatory protein antibody
    • KHSRP antibody
    • KSRP antibody
    • p75 antibody
    see all

Images

  • Western blot - Anti-KHSRP antibody (ab229660)
    Western blot - Anti-KHSRP antibody (ab229660)
    Anti-KHSRP antibody (ab229660) at 1/1000 dilution + Mouse brain tissue extract at 50 µg

    Predicted band size: 73 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)

    Paraffin-embedded rat deudenum tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)

    Paraffin-embedded mouse intestine tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemicall analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody (ab229660)
    Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody (ab229660)

    4% paraformaldehyde-fixed SK-N-AS (human neuroblastoma cell line) cells stained for KHSRP (green) using ab229660 at 1/400 dilution in immunohistochemical analysis.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Western blot - Anti-KHSRP antibody (ab229660)
    Western blot - Anti-KHSRP antibody (ab229660)
    All lanes : Anti-KHSRP antibody (ab229660) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa



    7.5% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab229660? Please let us know so that we can cite the reference in this datasheet.

    ab229660 has been referenced in 1 publication.

    • Käfer R  et al. The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells. J Immunol Res 2019:4726532 (2019). PubMed: 31511826

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab229660.
    Please use the links above to contact us or submit feedback about this product.

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