Key features and details
- Rabbit polyclonal to KHSRP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KHSRP antibody
See all KHSRP primary antibodies
DescriptionRabbit polyclonal to KHSRP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
Recombinant fragment within Human KHSRP (internal sequence). The exact sequence is proprietary.
Database link: Q92945
- WB: A431 and HeLa whole cell extracts; Mouse brain extract. ICC/IF: SK-N-AS cells. IHC/P: Mouse intestine tissue; Rat duodenum.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 73 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionBinds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Tissue specificityDetected in neural and non-neural cell lines.
Sequence similaritiesBelongs to the KHSRP family.
Contains 4 KH domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
- Information by UniProt
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
Anti-KHSRP antibody (ab229660) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 73 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded rat deudenum tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse intestine tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemicall analysis.
4% paraformaldehyde-fixed SK-N-AS (human neuroblastoma cell line) cells stained for KHSRP (green) using ab229660 at 1/400 dilution in immunohistochemical analysis.
Nuclear counterstain: Hoechst 33342 (blue).
All lanes : Anti-KHSRP antibody (ab229660) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229660 has been referenced in 1 publication.
- Käfer R et al. The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells. J Immunol Res 2019:4726532 (2019). PubMed: 31511826