Anti-KHSRP antibody (ab229660)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-KHSRP antibody
See all KHSRP primary antibodies -
Description
Rabbit polyclonal to KHSRP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken
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Immunogen
Recombinant fragment within Human KHSRP (internal sequence). The exact sequence is proprietary.
Database link: Q92945 -
Positive control
- WB: A431 and HeLa whole cell extracts; Mouse brain extract. ICC/IF: SK-N-AS cells. IHC/P: Mouse intestine tissue; Rat duodenum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 73 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs. -
Tissue specificity
Detected in neural and non-neural cell lines. -
Sequence similarities
Belongs to the KHSRP family.
Contains 4 KH domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites. - Information by UniProt
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Database links
- Entrez Gene: 374140 Chicken
- Entrez Gene: 8570 Human
- Entrez Gene: 16549 Mouse
- Entrez Gene: 171137 Rat
- Omim: 603445 Human
- SwissProt: Q92945 Human
- SwissProt: Q3U0V1 Mouse
- SwissProt: Q99PF5 Rat
see all -
Alternative names
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
see all
Images
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Western blot - Anti-KHSRP antibody (ab229660)Anti-KHSRP antibody (ab229660) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 73 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)Paraffin-embedded rat deudenum tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)Paraffin-embedded mouse intestine tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemicall analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody (ab229660)4% paraformaldehyde-fixed SK-N-AS (human neuroblastoma cell line) cells stained for KHSRP (green) using ab229660 at 1/400 dilution in immunohistochemical analysis.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-KHSRP antibody (ab229660)All lanes : Anti-KHSRP antibody (ab229660) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa7.5% SDS-PAGE gel.
References
ab229660 has not yet been referenced specifically in any publications.
