Anti-KHSRP antibody (ab229660)
Key features and details
- Rabbit polyclonal to KHSRP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-KHSRP antibody
See all KHSRP primary antibodies -
Description
Rabbit polyclonal to KHSRP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken
-
Immunogen
Recombinant fragment within Human KHSRP (internal sequence). The exact sequence is proprietary.
Database link: Q92945 -
Positive control
- WB: A431 and HeLa whole cell extracts; Mouse brain extract. ICC/IF: SK-N-AS cells. IHC/P: Mouse intestine tissue; Rat duodenum.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 73 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs. -
Tissue specificity
Detected in neural and non-neural cell lines. -
Sequence similarities
Belongs to the KHSRP family.
Contains 4 KH domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites. - Information by UniProt
-
Database links
- Entrez Gene: 374140 Chicken
- Entrez Gene: 8570 Human
- Entrez Gene: 16549 Mouse
- Entrez Gene: 171137 Rat
- Omim: 603445 Human
- SwissProt: Q92945 Human
- SwissProt: Q3U0V1 Mouse
- SwissProt: Q99PF5 Rat
see all -
Alternative names
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
see all
Images
-
Western blot - Anti-KHSRP antibody (ab229660)Anti-KHSRP antibody (ab229660) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 73 kDa7.5% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)Paraffin-embedded rat deudenum tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (ab229660)Paraffin-embedded mouse intestine tissue stained for KHSRP using ab229660 at 1/500 dilution in immunohistochemicall analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody (ab229660)4% paraformaldehyde-fixed SK-N-AS (human neuroblastoma cell line) cells stained for KHSRP (green) using ab229660 at 1/400 dilution in immunohistochemical analysis.
Nuclear counterstain: Hoechst 33342 (blue).
-
Western blot - Anti-KHSRP antibody (ab229660)All lanes : Anti-KHSRP antibody (ab229660) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab229660 has been referenced in 1 publication.
- Käfer R et al. The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells. J Immunol Res 2019:4726532 (2019). PubMed: 31511826
