Product nameAnti-KHSRP antibody
See all KHSRP primary antibodies
DescriptionRabbit polyclonal to KHSRP
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human KHSRP aa 660-710. The exact sequence is proprietary. Q92945.3
Database link: Q92945
- IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
FunctionBinds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Tissue specificityDetected in neural and non-neural cell lines.
Sequence similaritiesBelongs to the KHSRP family.
Contains 4 KH domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
- Information by UniProt
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
KHSRP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264265 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitates using a different KHSRP antibody at 1 μg/ml.
Lane 1: ab264265 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for KHSRP using ab264265 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264265 has not yet been referenced specifically in any publications.