Overview

  • Product name

    Anti-KHSRP antibody [EPR9864]
    See all KHSRP primary antibodies
  • Description

    Rabbit monoclonal [EPR9864] to KHSRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KHSRP aa 50-150. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, MCF7 and A673 cell lysates; Human brain and colon tissues; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9864
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/2000.

Target

  • Function

    Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
  • Tissue specificity

    Detected in neural and non-neural cell lines.
  • Sequence similarities

    Belongs to the KHSRP family.
    Contains 4 KH domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
  • Information by UniProt
  • Database links

  • Alternative names

    • Far upstream element-binding protein 2 antibody
    • FBP2 antibody
    • FUBP2 antibody
    • FUBP2_HUMAN antibody
    • FUSE binding protein 2 antibody
    • FUSE-binding protein 2 antibody
    • KH type splicing regulatory protein (FUSE binding protein 2) antibody
    • KH type splicing regulatory protein antibody
    • KH type-splicing regulatory protein antibody
    • KHSRP antibody
    • KSRP antibody
    • p75 antibody
    see all

Images

  • All lanes : Anti-KHSRP antibody [EPR9864] (ab150393) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A673 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat ani-Rabbit at 1/2000 dilution

    Predicted band size: 73 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling KHSRP with ab150393 at 1/100 dilution.
  • Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KHSRP (red) with purified ab150393 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling KHSRP with ab150393 at 1/100 dilution.
  • Immunofluorescent analysis of MCF7 cells labelling KHSRP with ab150393 at 1/100 dilution.

References

This product has been referenced in:

  • Zhao H  et al. KSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression. Nat Commun 8:1428 (2017). Read more (PubMed: 29127290) »
  • Yuan X  et al. USP39 promotes colorectal cancer growth and metastasis through the Wnt/ß-catenin pathway. Oncol Rep 37:2398-2404 (2017). Read more (PubMed: 28259917) »
See all 4 Publications for this product

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