Overview

  • Product name

    Anti-KHSRP antibody [EPR9865]
    See all KHSRP primary antibodies
  • Description

    Rabbit monoclonal [EPR9865] to KHSRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KHSRP aa 500-600. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, Jurkat and A673 cell lysates. Human brain and Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9865
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
  • Tissue specificity

    Detected in neural and non-neural cell lines.
  • Sequence similarities

    Belongs to the KHSRP family.
    Contains 4 KH domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
  • Information by UniProt
  • Database links

  • Alternative names

    • Far upstream element-binding protein 2 antibody
    • FBP2 antibody
    • FUBP2 antibody
    • FUBP2_HUMAN antibody
    • FUSE binding protein 2 antibody
    • FUSE-binding protein 2 antibody
    • KH type splicing regulatory protein (FUSE binding protein 2) antibody
    • KH type splicing regulatory protein antibody
    • KH type-splicing regulatory protein antibody
    • KHSRP antibody
    • KSRP antibody
    • p75 antibody
    see all

Images

  • All lanes : Anti-KHSRP antibody [EPR9865] (ab140648) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A673 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 73 kDa

  • Overlay histogram showing Hela cells stained with ab140648 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140648, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical analysis of paraffin embedded Human brain tissue labelling KHSRP with ab140648 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling KHSRP with ab140648 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140648 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140648 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140648 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab140648 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140648 showing +ve staining.

References

ab140648 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEF)
Total protein in input
6e+006 cells
Immuno-precipitation step
Protein A
Specification
MEF

Abcam user community

Verified customer

Submitted Jan 17 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
Yes - 0.3% Triton
Specification
MEF
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 15 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing (10% acrilamide)
Loading amount
20 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 15 2018

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (Human embryonic stem cell derived cardiomyocytes)
Specification
Human embryonic stem cell derived cardiomyocytes
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 15 2014

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