Recombinant Anti-Ki67 antibody [BLR021E] (ab243878)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR021E] to Ki67
- Suitable for: IHC-P, ICC, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ki67 antibody [BLR021E]
See all Ki67 primary antibodies -
Description
Rabbit monoclonal [BLR021E] to Ki67 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Ki67 aa 1200-1250. The exact sequence is proprietary. Corresponds to the repetitive 66 bp element. NP_002408.2 and Gene ID 4288.
Database link: P46013 -
Positive control
- IHC-P: Human tonsil and ovarian carcinoma tissue. ICC: OVCAR-4 cells. ICC/IF: Acetone/methanol fixed Hela cells. Flow Cyt: MOLT4 cells.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR021E -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
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ICC/IF |
1/100 - 1/500.
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Flow Cyt |
Use 1µl for 106 cells.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
1/100 - 1/500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
ICC/IF
1/100 - 1/500. |
Flow Cyt
Use 1µl for 106 cells. |
Target
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Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed. -
Sequence similarities
Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain. -
Developmental stage
Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815). -
Post-translational
modificationsPhosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. -
Cellular localization
Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). - Information by UniProt
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Database links
- Entrez Gene: 4288 Human
- Omim: 176741 Human
- SwissProt: P46013 Human
- Unigene: 689823 Human
- Unigene: 80976 Human
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Alternative names
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue, labeling Ki67 with ab243878. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Detection: DAB.
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Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-4 cells, labeling Ki67 with ab243878. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Detection: DAB.
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Immunocytochemical analysis of acetone/menthanol fixed HeLa cells, labeling Ki67 with ab243878 at a 1/100 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Counterstain: DAPI.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue, labeling Ki67 with ab243878 at a 1/500 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Substrate: Opal™. Counterstain: DAPI (blue).
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Flow cytometric analysis of 4% formaldehyde and permeabilized with 90% methanol MOLT-4 (Human lymphoblastic leukemia cell line) cells labelling Ki67 with ab243878 at 1µl/106 cells (Red shaded) compared with a Isotype control (unshaded). DyLight® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab243878 has been referenced in 9 publications.
- Jin M et al. Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis. Thorac Cancer 13:1276-1288 (2022). PubMed: 35348291
- Qi H et al. A Novel Role of IL13Rα2 in the Pathogenesis of Proliferative Vitreoretinopathy. Front Med (Lausanne) 9:831436 (2022). PubMed: 35770008
- Xu M et al. Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway. J Exp Clin Cancer Res 41:253 (2022). PubMed: 35986343
- Hu X et al. Knockdown of SLCO4C1 inhibits cell proliferation and metastasis in endometrial cancer through inactivating the PI3K/Akt signaling pathway. Oncol Rep 43:919-929 (2020). PubMed: 32020231
- Dou D et al. CircUBE2D2 (hsa_circ_0005728) promotes cell proliferation, metastasis and chemoresistance in triple-negative breast cancer by regulating miR-512-3p/CDCA3 axis. Cancer Cell Int 20:454 (2020). PubMed: 32944002
- Chen L et al. Silencing transcription factor FOXM1 represses proliferation, migration, and invasion while inducing apoptosis of liver cancer stem cells by regulating the expression of ALDH2. IUBMB Life 72:285-295 (2020). PubMed: 31580537
- Yan C et al. LncRNA HULC shRNA disinhibits miR-377-5p to suppress the growth and invasion of hepatocellular carcinoma in vitro and hepatocarcinogenesis in vivo. Ann Transl Med 8:1294 (2020). PubMed: 33209874
- Wang Z et al. KLF5 influences cell biological function and chemotherapy sensitivity through the JNK signaling pathway in anaplastic thyroid carcinoma. J Biochem Mol Toxicol 34:e22469 (2020). PubMed: 32173973
- Lixin S et al. miR-885-5p inhibits proliferation and metastasis by targeting IGF2BP1 and GALNT3 in human intrahepatic cholangiocarcinoma. Mol Carcinog 59:1371-1381 (2020). PubMed: 33052627