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    ki67-antibody-epr3610-ab92742.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ki67 antibody [EPR3610] (ab92742)

  • Datasheet
  • SDS
Reviews (12)Q&A (2)References (133)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Western blot - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Flow Cytometry - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Flow Cytometry - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Western blot - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
  • Anti-Ki67 antibody [EPR3610] (ab92742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3610] to Ki67
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Ki67 antibody [EPR3610]
    See all Ki67 primary antibodies
  • Description

    Rabbit monoclonal [EPR3610] to Ki67
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICCmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Ki67 aa 1050-1150. The exact sequence is proprietary.
    Database link: P46013-1

  • Positive control

    • WB: HeLa and ramos cell lysates. IHC-P: Human tonsil, colon, ovarian carcinoma, squamous cell carcinoma of cervix and colonic adenocarcinoma tissues. ICC: HeLa, HT-29 cells, HAP1 cells. Flow Cyt: Ramos cells.
  • General notes

    This product is not suitable for xenograft experiments. For further information please contact our Customer Services team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.24 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3610
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (ab196907)
    • Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
    • Anti-Ki67 antibody [EPR3610] - BSA and Azide free (ab209897)
    • Alexa Fluor® 568 Anti-Ki67 antibody [EPR3610] (ab211968)
    • HRP Anti-Ki67 antibody [EPR3610] (ab212215)
    • Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226)
    • Alexa Fluor® 594 Anti-Ki67 antibody [EPR3610] (ab216709)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Ramos whole cell lysate (ab3955)
  • Related Products

    • Ki67 peptide (ab15581)

Applications

Our Abpromise guarantee covers the use of ab92742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 359 kDa.

For unpurified use at 1/500 - 1/1000.

IHC-P 1/500 - 1/1000.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

Antigen retrieval: Heat in citrate buffer pH 6 for 20-30 min or enzymatic (trypsin, proteinase K) necessary if fixed in PFA.

Positive Control: Hu tonsil tissue, Ms - tumour, embryonic skin tissue, Rt - esophagus, small intestine and liver tissue

Flow Cyt 1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use a concentration of 1 µg/ml.

For nuclear proteins, fixing cells in 4% PFA (20 min, room temp) is recommended.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen.

Positive Control: HeLa and HAP1 cells / Rt cardiomyocytes

Target

  • Function

    Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
  • Sequence similarities

    Contains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.
  • Developmental stage

    Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
  • Post-translational
    modifications

    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
  • Cellular localization

    Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Target information above from: UniProt accession P46013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4288 Human
    • Omim: 176741 Human
    • SwissProt: P46013 Human
    • Unigene: 689823 Human
    • Unigene: 80976 Human
    • Alternative names

      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)Ishii et al PLoS One. 2016 Jan 22;11(1):e0147366. doi: 10.1371/journal.pone.0147366. eCollection 2016

      Comparison between RNApII-S2P-/low cells and Ki-67- cells

      a: Regulation of Ki-67 and RNApII-S2P during proliferation and quiescence in T98G glioblastoma cells. T98G cells were grown in culture medium containing 10% (v/v) fetal bovine serum (FBS), were induced to become quiescent by serum starvation in medium supplemented with 0.5% (v/v) FBS for 14 days, and then were re-stimulated by being split 1:5 into new medium containing 10% (v/v) FBS and cultured for 3 days. The cells were detached from dishes with trypsin-EDTA solution, fixed in 10% (v/v) neutral buffered formalin, and centrifuged. Paraffin sections of the pellet were cut, and expression of Ki-67 and RNApII-S2P was examined by single (brown; a1, a2, a4, a5, a7, a8) or double immunostaining (Ki-67, brown; RNApII-S2P, red; a3, a6, a9). Hematoxylin (blue) was used as a nuclear stain. Ki-67- RNApII-S2P-/low cells (blue cells in the double stained sections) emerged only in the quiescent condition (a6, arrows). Scale bar, 10 μm. b: Single-color immunostaining for Ki-67 (b1) and RNApII-S2P (b2) in serial sections of glioblastoma tissue. Ki-67- tumor cells were frequently found, whereas only a few RNApII-S2P-/low cells (arrows) were observed around necrotic area. N, necrotic area; V, blood vessels. Scale bars, 50 μm.

      Ki67 detected using ab92742.

      (From Figure S2 of Ishii et al)

    • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)

      ab92742 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab92742 at 1µg/ml and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labeled in blue with DAPI.

      Alexa Fluor® 488 (ab197234) and Alexa Fluor® 647 (ab196907) conjugated versions are available for this clone.

    • Western blot - Anti-Ki67 antibody [EPR3610] (ab92742)
      Western blot - Anti-Ki67 antibody [EPR3610] (ab92742)
      Anti-Ki67 antibody [EPR3610] (ab92742) at 1/5000 dilution (purified) + Ramos (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 359 kDa
      Observed band size: 395 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry - Anti-Ki67 antibody [EPR3610] (ab92742)
      Flow Cytometry - Anti-Ki67 antibody [EPR3610] (ab92742)

      Overlay histogram showing Ramos (Human Burkitt's lymphoma cell line) cells stained with unpurified ab92742 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab92742, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions.

      Acquisition of >5,000 events was performed.

      Alexa Fluor® 488 (ab197234) and Alexa Fluor® 647 (ab196907) conjugated versions are available for this clone.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cell carcinoma of cervix tissue labeling Ki67 with purified ab92742 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

      Negative control using PBS instead of primary antibody (inset).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)Ishii et al PLoS One. 2016 Jan 22;11(1):e0147366. doi: 10.1371/journal.pone.0147366. eCollection 2016. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Chromogenic triple immunostaining for estrogen receptor (ER), progesterone receptor (PgR), and Ki-67 in breast cancer tissue to verify the triple immunostaining detection method.

      Panel e: ER+ PgR- Ki-67- cells were stained red (short arrow), ER- PgR+ Ki-67- cells were stained blue (black arrowhead), ER+ PgR+ Ki-67- cells were stained purple (long arrow), and Ki-67+ cells were stained brown (white arrowhead). These colors are easily distinguishable. Scale bars, 25 μm.

      Deparaffinized sections were pretreated for antigen retrieval by boiling in antigen retrieval solution, pH 9. Sections were incubated with rabbit monoclonal antibody against Ki67 ab92742 at a 1/1000 dilution. After the reaction with (HRP)-conjugated secondary antibodies color was developed with (DAB) and sections were counterstained with hematoxylin.

    • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunocytochemistry analysis of HT-29 (Human colorectal adenocarcinoma cell line) cells labeling Ki67 with purified ab92742 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal colon tissue labeling Ki67 with unpurified ab92742. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labeling Ki67 with unpurified ab92742. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Ki67 antibody [EPR3610] (ab92742)
      Flow Cytometry - Anti-Ki67 antibody [EPR3610] (ab92742)

      Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma cell line) cells lablling Ki67 with purified ab92742 at 1/150 (red). Cells were fixed with 2% paraformaldehyde. An FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labeling Ki67 with unpurified ab92742. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Western blot - Anti-Ki67 antibody [EPR3610] (ab92742)
      Western blot - Anti-Ki67 antibody [EPR3610] (ab92742)
      Anti-Ki67 antibody [EPR3610] (ab92742) at 1/500 dilution (unpurified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 359 kDa
      Observed band size: 395 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling Ki67 with unpurified ab92742. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)This image is courtesy of an anonymous Abreview.

      ab92742 staining Ki67 in human adenocarcinoma cells by ICC (Immunocytochemistry).

      Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A Cy3® conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/200.

      See Abreview

    • Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)
      Immunocytochemistry - Anti-Ki67 antibody [EPR3610] (ab92742)

      Immunocytochemistry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Ki67 with unpurified ab92742 at a dilution of 1/250.

    • Anti-Ki67 antibody [EPR3610] (ab92742)
      Anti-Ki67 antibody [EPR3610] (ab92742)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (133)

    Publishing research using ab92742? Please let us know so that we can cite the reference in this datasheet.

    ab92742 has been referenced in 133 publications.

    • Xiao X  et al. Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer. Oncotarget 11:1203-1217 (2020). PubMed: 32292571
    • Lan W  et al. Targeting NUPR1 with the small compound ZZW-115 is an efficient strategy to treat hepatocellular carcinoma. Cancer Lett 486:8-17 (2020). PubMed: 32446862
    • Wang J  et al. Knockdown of long non-coding RNA PVT1 induces apoptosis of fibroblast-like synoviocytes through modulating miR-543-dependent SCUBE2 in rheumatoid arthritis. J Orthop Surg Res 15:142 (2020). PubMed: 32293498
    • Zhong W  et al. Knockdown of lncRNA PVT1 alleviates high glucose-induced proliferation and fibrosis in human mesangial cells by miR-23b-3p/WT1 axis. Diabetol Metab Syndr 12:33 (2020). PubMed: 32322310
    • Liu D  et al. METTL7B Is Required for Cancer Cell Proliferation and Tumorigenesis in Non-Small Cell Lung Cancer. Front Pharmacol 11:178 (2020). PubMed: 32180726
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [EPR3610]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ovary Carcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Ovary Carcinoma
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 08 2018

    Immunocytochemistry abreview for Anti-Ki67 antibody [EPR3610]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Chicken Cell (human HCT116 in Chicken CAM)
    Permeabilization
    Yes - 0,5% Triton X-100
    Specification
    human HCT116 in Chicken CAM
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 24 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Ki67 antibody [EPR3610]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (brain organoid)
    Permeabilization
    Yes - PBS-Triton 0.5%
    Specification
    brain organoid
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Kalina Draganova

    Verified customer

    Submitted Sep 11 2017

    Immunocytochemistry abreview for Anti-Ki67 antibody [EPR3610]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Adenocarcinoma cells)
    Permeabilization
    Yes - 0.1% TX-100
    Specification
    Adenocarcinoma cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 13 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [EPR3610]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [EPR3610]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 25 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [EPR3610]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 19 2015

    Flow Cytometry abreview for Anti-Ki67 antibody [EPR3610]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 15 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [EPR3610]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 18 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Ki67 antibody [EPR3610]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Human Glioma into mouse brain)
    Permeabilization
    Yes - Triton X-100
    Specification
    Human Glioma into mouse brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 27 2015

    1-10 of 14 Abreviews or Q&A

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