Anti-Ki67 antibody - Proliferation Marker, prediluted (ab15467)


  • Product name
    Anti-Ki67 antibody - Proliferation Marker, prediluted
    See all Ki67 primary antibodies
  • Description
    Rabbit polyclonal to Ki67 - Proliferation Marker, prediluted
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Positive control
    • Breast carcinoma and lymph node.



Our Abpromise guarantee covers the use of ab15467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: Ready to use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
    • Sequence similarities
      Contains 1 FHA domain.
      Contains 16 K167R repeats.
      Contains 1 PP1-binding domain.
    • Developmental stage
      Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
    • Post-translational
      Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
    • Cellular localization
      Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all


    • ab15467 staining Ki67 in human lymph node by Immunohistochemistry (FFPE-sections).


    This product has been referenced in:
    • Canbay E  et al. Dual inhibition of wound healing and oxidative process by montelukast in experimental colon anastomoses. Surg Innov 17:248-55 (2010). IHC-P ; Rat . Read more (PubMed: 20634223) »
    • Colin C  et al. Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis. Neuropathol Appl Neurobiol 33:431-9 (2007). Read more (PubMed: 17442061) »
    See all 2 Publications for this product

    Customer reviews and Q&As


    Thank you for your inquiry.

    Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted antibodies are used neat there might be reasons to use 50ul per slide or maybe even 100ul per slide.

    These conditions include: size of the sample, tissue type, lab experience, diluent buffer, species, antigen retrieval, incubation time, etc.

    For the products you mentioned I can give a estimate for the pre-diluted antibodies of ca 140- 280slides, for the other antibodies 500 - 100 slides and for the detection kit 150 - 300 slides.

    I hope this information is helpful.

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