Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [SP6] - Proliferation Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Small Intestine)
Specification
Small Intestine
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C

Other product details

Incubation time
30 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit biotinlated secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/2000

Additional data

Additional Notes
Ki67 (ab16667) used at 1:100 dilution and for 30 minute incubation at room temperature.

Jing Ma

Verified customer

Submitted Mar 15 2011

Sign up