Western blot abreview for Anti-Ki67 antibody [SP6]

Inconclusive
Abreviews
Application
Western blot
Sample
Cow Tissue lysate - whole (Forestomach (rumen, reticulum, omasum, abomasum))
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
10 µg
Specification
Forestomach (rumen, reticulum, omasum, abomasum)
Blocking step
(agent) for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Casein in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/2000

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Positive control
L-WRN cell lysate(Mouse), a band observed at >245 kDa (might be specific band)

Additional data

Abcam response
Thank you so much for testing this antibody in cow. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species. We welcome additional feedback from other customers as well.

Abcam user community

Verified customer

Submitted Mar 07 2018

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