Immunocytochemistry/ Immunofluorescence abreview for Anti-Ki67 antibody [SP6]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Lung Epithelial cells)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Lung Epithelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBSA

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti Rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The image is ki67 (alexa 488) and dapi (blue) many of the nuclei cells positive (green staining) for example the cell in the centre rigjt of the image has bright green staining.
Abcam response
no green color, though secondary is green in color

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Submitted May 22 2018

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