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    ki67-antibody-sp6-bsa-and-azide-free-ab197547.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

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Western blot - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Multiplex immunohistochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Flow Cytometry (Intracellular) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
  • Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP6] to Ki67 - BSA and Azide free
  • Suitable for: ICC, WB, mIHC, Flow Cyt (Intra), IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Ki67 antibody [SP6] - BSA and Azide free
    See all Ki67 primary antibodies
  • Description

    Rabbit monoclonal [SP6] to Ki67 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, mIHC, Flow Cyt (Intra), IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Common marmoset
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    C-terminus
  • Positive control

    • WB: HeLa cell lysate. IHC-P: Human tonsil and testis tissue. Common marmoset spleen tissue. Rat esophagus, small intestine and liver tissue. Mouse embryonic skin tissue. IHC-Fr: Rat lymph node tissue, Transgenic mouse spinal cord tissue. ICC: HeLa and HAP1 cells. Human cardiac stem cells. HEp-2 cells. Rat cardiomyocytes. Flow Cyt (intra): HAP1 cells.
  • General notes

    ab197547 is the carrier-free version of ab16667.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    SP6
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-Ki67 antibody [SP6] (ab16667)
    • Anti-Ki67 antibody [SP6], prediluted (ab21700)
    • Anti-Ki67 antibody [SP6] - BSA free (ab231172)
    • Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847)
    • Alexa Fluor® 647 Anti-Ki67 antibody [SP6] (ab281928)
    • PE Anti-Ki67 antibody [SP6] (ab282173)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/50.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.

 

WB
Use at an assay dependent concentration. Predicted molecular weight: 358 kDa.
mIHC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P (1)
1/100.

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Primary antibody condition: primary antibody incubation overnight at +4°C is recommended.

Notes
ICC
1/50.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.

 

WB
Use at an assay dependent concentration. Predicted molecular weight: 358 kDa.
mIHC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
1/100.

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Primary antibody condition: primary antibody incubation overnight at +4°C is recommended.

Target

  • Function

    Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
  • Sequence similarities

    Contains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.
  • Developmental stage

    Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
  • Post-translational
    modifications

    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
  • Cellular localization

    Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Target information above from: UniProt accession P46013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4288 Human
    • Entrez Gene: 17345 Mouse
    • Entrez Gene: 246042 Rat
    • Omim: 176741 Human
    • SwissProt: P46013 Human
    • SwissProt: E9PVX6 Mouse
    • SwissProt: Q61769 Mouse
    • Unigene: 689823 Human
    • Unigene: 80976 Human
    • Unigene: 4078 Mouse
    see all
  • Alternative names

    • Antigen identified by monoclonal antibody Ki 67 antibody
    • Antigen identified by monoclonal antibody Ki-67 antibody
    • Antigen KI-67 antibody
    • Antigen KI67 antibody
    • Antigen Ki67 antibody
    • KI67_HUMAN antibody
    • KIA antibody
    • Marker of proliferation Ki-67 antibody
    • MIB 1 antibody
    • MIB antibody
    • MKI67 antibody
    • PPP1R105 antibody
    • Proliferation marker protein Ki-67 antibody
    • Proliferation related Ki 67 antigen antibody
    • Protein phosphatase 1 regulatory subunit 105 antibody
    • RP11-380J17.2 antibody
    see all

Images

  • Western blot - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Western blot - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    All lanes : Anti-Ki67 antibody [SP6] (ab16667) at 1/100 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Wild-type HeLa cell lysate
    Lane 3 : MKI67 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 358 kDa
    Observed band size: 359 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

    Lanes 1-3: Merged signal (red and green). Green - ab16667 observed at 359 kDa. Red - loading control, ab130007 observed at 125 kDa.

    ab16667 was shown to react with Ki67 in wild-type HeLa. Loss of signal was observed when knockout sample ab263762 was used. Wild-type and Ki67 knockout samples were subjected to SDS-PAGE. ab16667 and Anti-Vinculin antibody VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 100 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)  and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil sections labeling Ki67 with ab16667 at 1/200 (0.156 µg/mL). 

    Image A: 
    Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer)
    Human tonsil tissue incubated with ab16667 overnight at +4°C.
    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
    Nuclear staining on human tonsil. The section was incubated with ab16667 overnight at +4°C.

    Image B:
    Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
    Human tonsil tissue incubated with ab16667 on a Leica Biosystems BOND® RX instrument.
    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Multiplex immunohistochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

     

    Fluorescence multiplex immunohistochemical analysis of normal human tonsil tissue (formalin-fixed paraffin-embedded section).

    Merged staining of anti-PD1 (ab237728; orange; Opal™520), anti-PDL1 (ab237726; green; Opal™540), anti-CD68 (ab192847; yellow; Opal™570), anti-CD3 (ab16669; red; Opal™620), anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®).

    The section was incubated in six rounds of staining; in the order of ab237728 (1/500 dilution), ab237726 (1/500 dilution), ab192847 (1/300 dilution), ab16669 (1/300 dilution), ab16667 (1/200 dilution) and ab7753 (1/200 dilution); each using a separate fluorescent tyramide signal amplification system.

    Sodium citrate antigen retrieval (Leica ER1, pH6.0, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

    DAPI (dark blue) was used as a nuclear counter stain.

    Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra Polaris.

    This image was generated from the hybridoma version.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

    IHC image of ab16667 staining Ki67 in a section of formalin-fixed paraffin-embedded Rat spleen tissue. The section was pre-treated using heat mediated antigen retrieval with (citrate buffer, pH 6.0). The section was then incubated with ab16667, 1/200 (0.156 µg/mL) dilution and detected using ready to use Goat Anti-Rabbit IgG H&L (HRP) antibody. Nuclear staining on rat spleen. The section was incubated with ab16667 overnight at +4°C. DAB was used as the chromogen. The section was then counterstained with haematoxylin. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

    IHC image of ab16667 staining Ki67 in a section of formalin-fixed paraffin-embedded Human tonsil tissue. The section was pre-treated using heat mediated antigen retrieval with ab93678 (citrate buffer, pH 6.0). The section was then incubated with ab16667, 1/200 (0.156 µg/ml) dilution and detected using ready to use Goat Anti-Rabbit IgG H&L (HRP) antibody. Nuclear staining on human tonsil is observed. The section was incubated with ab16667 overnight at +4°C. DAB was used as the chromogen. The section was then counterstained with haematoxylin. 

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

    IHC image of ab16667 staining Ki67 in a section of formalin-fixed paraffin-embedded Mouse spleen tissue. The section was pre-treated using heat mediated antigen retrieval with ab93678 (citrate buffer, pH 6.0). The section was then incubated with ab16667, 1/200 (0.156 µg/mL) dilution and detected using a ready to use Goat Anti-Rabbit IgG H&L (HRP) antibody. Nuclear staining on mouse spleen.The section was incubated with ab16667 overnight at +4°C. DAB was used as the chromogen. The section was then counterstained with haematoxylin. 

  • Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

     

    ab16667 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab16667 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    This image was generated from the hybridoma version.

  • Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using ab16667, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, None permeabilized parental HAP1 cells labelling Ki67 with ab16667 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nucleolar staining in parental HAP1cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).

  • Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunocytochemistry - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using ab16667, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, None permeabilized HeLa cells labelling Ki67 with ab16667 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nucleolar staining in HeLa cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

     

    IHC image of ab16667 staining Ki67 in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. The section was then incubated with ab16667, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This image was generated from the hybridoma version.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

     

    ab16667 staining Ki67 in rat oesophagus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% BSA for 10 minutes at 21ºC followed by incubation with the primary antibody for 30 minutes at 1/100. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    This image was generated from the hybridoma version.

  • Flow Cytometry (Intracellular) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Flow Cytometry (Intracellular) - Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

    This data was developed using ab16667, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) cells labelling Ki67 with ab16667 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

     

  • Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)
    Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab197547? Please let us know so that we can cite the reference in this datasheet.

ab197547 has been referenced in 2 publications.

  • Si H  et al. Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis. Cancer Manag Res 13:1099-1111 (2021). PubMed: 33574707
  • Zhan Y  et al. Knockdown of Long Non-Coding RNA HOTAIR Suppresses Cisplatin Resistance, Cell Proliferation, Migration and Invasion of DDP-Resistant NSCLC Cells by Targeting miR-149-5p/Doublecortin-Like Kinase 1 Axis. Cancer Manag Res 12:7725-7737 (2020). PubMed: 32943921

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [SP6] - BSA and Azide free

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
Yes - Tween, Triton
Specification
Lymph node
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 17 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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