Product nameAnti-KIAA0020 antibody
See all KIAA0020 primary antibodies
DescriptionRabbit polyclonal to KIAA0020
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset
Synthetic peptide corresponding to a region within amino acids 1-50 of Human KIAA0020 (NP_055693.4).
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab156692 was affinity purified using an epitope specific to KIAA0020 immobilized on solid support.
Our Abpromise guarantee covers the use of ab156692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 74 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 PUM-HD domain.
Contains 6 pumilio repeats.
DomainThe HA-8 region can be cleaved and exposed at the cell surface where it plays a role as a minor histocompatibility HLA-A*0201-restricted antigen.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- HA-8 antibody
- HBV X transactivated gene 5 protein antibody
- HBV X-transactivated gene 5 protein antibody
All lanes : Anti-KIAA0020 antibody (ab156692) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes
Detection of KIAA0020 by Western blot of immunoprecipitate.
Immunoprecipitation of KIAA0020 (6 µg of antibody per mg of HeLa whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: ab156692
Lane 2: alternative anti-KIAA0020 antibody
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156692 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lane 1 : Anti-KIAA0020 antibody (ab156692) at 1 µg/ml
Lane 2 : Alternative rabbit anti-KIAA0020 antibody
Lane 3 : Control IgG
All lanes : HeLa whole cell lysate
ab156692 has not yet been referenced specifically in any publications.