Key features and details
- Rabbit polyclonal to KIAA0355
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KIAA0355 antibody
DescriptionRabbit polyclonal to KIAA0355
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen sequence, corresponding to internal sequence amino acids 379-482 of Human KIAA0355.
- Human small intestine tissue. RT-4 and U-251 MG cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
- Information by UniProt
- K0355_HUMAN antibody
- KIAA0355 antibody
- Uncharacterized protein KIAA0355 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121441 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121441 at 1/20 dilution staining KIAA0355 in paraffin-embedded Human small intestine tissue by Immunohistochemistry.
All lanes : Anti-KIAA0355 antibody (ab121441) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
ab121441 has not yet been referenced specifically in any publications.