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Immunogen was a synthetic peptide, which represented a portion of human hypothetical protein KIAA0460 encoded within the 5-prime end of exon 11 (LocusLink ID 23248).
Our Abpromise guarantee covers the use of ab10363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 220 kDa (predicted molecular weight: 160 kDa).|
ab10363 detection of human KIAA0460 by Western blot. Samples: Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody: ab10363 used at 1 mg/ml. Detection: Chemiluminescence with 3 minute exposure.
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