Overview

  • Product name
  • Description
    Goat polyclonal to KIAA0460
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human hypothetical protein KIAA0460 encoded within the 5-prime end of exon 11 (LocusLink ID 23248).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 220 kDa (predicted molecular weight: 160 kDa).

Target

  • Relevance
    This is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • FLJ 32145 antibody
    • HSPC 099 antibody
    • HSPC099 antibody
    • Hypothetical Protein DKFZp686H22247 antibody
    • Hypothetical protein KIAA0460 antibody
    • KIAA 0460 antibody
    • Regulation of nuclear pre mRNA domain containing protein 2 antibody
    • Regulation of nuclear pre-mRNA domain containing 2 antibody
    • RPRD 2 antibody
    • RPRD2 antibody
    see all

Images

  • ab10363 detection of human KIAA0460 by Western blot. Samples: Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody: ab10363 used at 1 mg/ml. Detection: Chemiluminescence with 3 minute exposure.

    ab10363 detection of human KIAA0460 by Western blot. Samples: Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody: ab10363 used at 1 mg/ml. Detection: Chemiluminescence with 3 minute exposure.

References

This product has been referenced in:
  • Lv XJ  et al. RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol Rep : (2016). WB ; Mouse . Read more (PubMed: 26782095) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your interest. The precise peptides used in the production of the antibodies ab10363 and ab10373 to KIAA0460 are proprietary. The antibody ab10363 is to a peptide that is encoded within the 5’ end of exon 11. The antibody ab10373 is to a peptide that is encoded within the 3’ end of exon 11. According to NCBI’s evidence viewer: (http://www.ncbi.nlm.nih.gov/sutils/evv.cgi?contig=NT_032962.5&gene=KIAA0460&lid=23248), Exon 11 encodes residues 529 to 1454 of BAA32305.2. The apparent MW on SDS-PAGE gels under the conditions in which the antibodies to KIAA0460 were tested is slightly larger than 220 kDa. Supporting evidence for this are: 1) both antibodies recognize a protein of the same Mr despite being generated to peptides that have nothing in common other than both are unique to KIAA0460 and 2) the antibodies were generated after KIAA0460 was identified as a protein that co-immunoprecipited with proteins that are established to be involved in repair of DNA damage. The gel slice, from which KIAA0460 was identified in this complex, was taken from the 220 to 240 Mr region of the gel. Owing to contractual obligations, the complex from which KIAA0460 was identified cannot be divulged prior to publication of these data.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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