Product nameAnti-KIAA0460 antibody
See all KIAA0460 primary antibodies
DescriptionGoat polyclonal to KIAA0460
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HEK293 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to KIAA0460 immobilized on solid support.
Our Abpromise guarantee covers the use of ab17996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 155 kDa).|
|IP||Use at an assay dependent dilution.|
RelevanceThis is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.
- FLJ 32145 antibody
- HSPC 099 antibody
- HSPC099 antibody
Lanes 1-2 : Anti-KIAA0460 antibody (ab17996) at 1 µg/ml
Lanes 3-4 : Anti-KIAA0460 antibody (ab17996) at 0.4 µg/ml
Lanes 1 & 3 : Whole cell lysate from HEK239 cells at 15 µg
Lanes 2 & 4 : Whole cell lysate from HEK239 cells at 50 µg
Predicted band size: 155 kDa
Observed band size: 225 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Detection of Human KIAA0460 by Western Blot.
Samples: Whole cell lysate (15 and 50
µg) from HEK293 cells.
Antibody: Affinity purified goat anti-KIAA0460 antibody used at 1 and 0.4 mg/ml.
Detection: Chemiluminescence with an exposure time of 1 minute.
ab17996 has not yet been referenced specifically in any publications.