Product nameAnti-KIAA0513 antibody
See all KIAA0513 primary antibodies
DescriptionRabbit polyclonal to KIAA0513
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
antigen, corresponding to amino acids 134-271 of Human KIAA0513.
- KIAA0513-transfected HEK293T lysate; Human cerebellum tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Tissue specificityWidely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.
- Information by UniProt
- K0513_HUMAN antibody
- KIAA0513 antibody
- Uncharacterized protein KIAA0513 antibody
All lanes : Anti-KIAA0513 antibody (ab121382) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : KIAA0513-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121382 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121382 at 1/500 dilution staining KIAA0513 in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry. Note: strong cytoplasmic and nuclear positivity in Purkinje cells and strong cytoplasmic positivity in cells in granular layer and molecular layer.
ab121382 has not yet been referenced specifically in any publications.