Anti-KIAA0513 antibody (ab121382)
Key features and details
- Rabbit polyclonal to KIAA0513
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIAA0513 antibody
See all KIAA0513 primary antibodies -
Description
Rabbit polyclonal to KIAA0513 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 134-271 of Human KIAA0513.
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Positive control
- KIAA0513-transfected HEK293T lysate; Human cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Tissue specificity
Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9764 Human
- Omim: 611675 Human
- SwissProt: O60268 Human
- Unigene: 301658 Human
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Alternative names
- K0513_HUMAN antibody
- KIAA0513 antibody
- Uncharacterized protein KIAA0513 antibody
Images
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Western blot - Anti-KIAA0513 antibody (ab121382)All lanes : Anti-KIAA0513 antibody (ab121382) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : KIAA0513-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KIAA0513 antibody (ab121382)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121382 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0513 antibody (ab121382)ab121382 at 1/500 dilution staining KIAA0513 in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry. Note: strong cytoplasmic and nuclear positivity in Purkinje cells and strong cytoplasmic positivity in cells in granular layer and molecular layer.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121382 has not yet been referenced specifically in any publications.