Anti-KIAA0513 antibody (ab121382)

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Overview

  • Product name

    Anti-KIAA0513 antibody
    See all KIAA0513 primary antibodies

  • Description

    Rabbit polyclonal to KIAA0513

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen, corresponding to amino acids 134-271 of Human KIAA0513.

  • Positive control

    • KIAA0513-transfected HEK293T lysate; Human cerebellum tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificity

    Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • K0513_HUMAN antibody
      • KIAA0513 antibody
      • Uncharacterized protein KIAA0513 antibody

    Images

    • Western blot - Anti-KIAA0513 antibody (ab121382)
      Western blot - Anti-KIAA0513 antibody (ab121382)
      All lanes : Anti-KIAA0513 antibody (ab121382) at 1/250 dilution

      Lane 1 : vector only transfected HEK293T lysate
      Lane 2 : KIAA0513-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

      Developed using the ECL technique.


      10-20% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-KIAA0513 antibody (ab121382)
      Immunocytochemistry/ Immunofluorescence - Anti-KIAA0513 antibody (ab121382)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121382 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0513 antibody (ab121382)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0513 antibody (ab121382)
      ab121382 at 1/500 dilution staining KIAA0513 in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry. Note: strong cytoplasmic and nuclear positivity in Purkinje cells and strong cytoplasmic positivity in cells in granular layer and molecular layer.

    References

    ab121382 has not yet been referenced specifically in any publications.

    Publishing research using ab121382? Please let us know so that we can cite the reference in this datasheet.

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    Western blot abreview for Anti-KIAA0513 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (cerebellum, cortical, hippocampal)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    cerebellum, cortical, hippocampal
    Blocking step
    1x Odyssey blocking buffer(OBB) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
    Read More

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    Verified customer

    Submitted Nov 30 2016

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