Anti-KIAA0513 antibody (ab121430)

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Overview

  • Product name

    Anti-KIAA0513 antibody
    See all KIAA0513 primary antibodies

  • Description

    Rabbit polyclonal to KIAA0513

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    N terminal amino acids 7-123 of Human KIAA0513.

  • Positive control

    • Human skeletal muscle tissue; RT-4 and Human liver tissue lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificity

    Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • K0513_HUMAN antibody
      • KIAA0513 antibody
      • Uncharacterized protein KIAA0513 antibody

    Images

    • Western blot - Anti-KIAA0513 antibody (ab121430)
      Western blot - Anti-KIAA0513 antibody (ab121430)
      All lanes : Anti-KIAA0513 antibody (ab121430) at 1/250 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human plasma
      Lanes 4-5 : Human Liver tissue lysate

      Developed using the ECL technique.
    • Immunocytochemistry/ Immunofluorescence - Anti-KIAA0513 antibody (ab121430)
      Immunocytochemistry/ Immunofluorescence - Anti-KIAA0513 antibody (ab121430)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121430 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0513 antibody (ab121430)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0513 antibody (ab121430)
      ab121430, at a 1/45 dilution, staining KIAA0513 in paraffin-embedded Human skeletal muscle, by Immunohistochemistry.

    References

    ab121430 has not yet been referenced specifically in any publications.

    Publishing research using ab121430? Please let us know so that we can cite the reference in this datasheet.

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