Key features and details
- Rabbit polyclonal to KIAA0513
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KIAA0513 antibody
See all KIAA0513 primary antibodies
DescriptionRabbit polyclonal to KIAA0513
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
N terminal amino acids 7-123 of Human KIAA0513.
- Human skeletal muscle tissue; RT-4 and Human liver tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Tissue specificityWidely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.
- Information by UniProt
- K0513_HUMAN antibody
- KIAA0513 antibody
- Uncharacterized protein KIAA0513 antibody
All lanes : Anti-KIAA0513 antibody (ab121430) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lanes 4-5 : Human Liver tissue lysate
Developed using the ECL technique.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121430 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121430, at a 1/45 dilution, staining KIAA0513 in paraffin-embedded Human skeletal muscle, by Immunohistochemistry.
ab121430 has not yet been referenced specifically in any publications.