Key features and details
- Rabbit polyclonal to KIAA0513
- Suitable for: WB, ICC/IF
- Isotype: IgG
Product nameAnti-KIAA0513 antibody
See all KIAA0513 primary antibodies
DescriptionRabbit polyclonal to KIAA0513
Tested applicationsSuitable for: WB, ICC/IFmore details
Recombinant fragment corresponding to Human KIAA0513 aa 1-301.
Database link: O60268
- WB: Jurkat, K562 whole cell lysate; Mouse brain tissue lysate. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 47 kDa.|
|ICC/IF||1/50 - 1/200.|
Tissue specificityWidely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.
- Information by UniProt
- K0513_HUMAN antibody
- KIAA0513 antibody
- Uncharacterized protein KIAA0513 antibody
All lanes : Anti-KIAA0513 antibody (ab236750) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : Mouse brain tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling KIAA0513 (green) using ab236750 at 1/100 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236750 has not yet been referenced specifically in any publications.