Overview

  • Product name

  • Description

    Rabbit polyclonal to KIAA0513
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Immunogen

    Recombinant fragment corresponding to Human KIAA0513 aa 1-301.
    Database link: O60268

  • Positive control

    • WB: Jurkat, K562 whole cell lysate; Mouse brain tissue lysate. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 47 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Tissue specificity

    Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • K0513_HUMAN antibody
    • KIAA0513 antibody
    • Uncharacterized protein KIAA0513 antibody

Images

  • All lanes : Anti-KIAA0513 antibody (ab236750) at 1/2000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : Mouse brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 47 kDa

  • HepG2 (human liver hepatocellular carcinoma cell line) cells labeling KIAA0513 (green) using ab236750 at 1/100 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

References

ab236750 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236750.
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