Key features and details
- Rabbit polyclonal to KIAA0528
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KIAA0528 antibody
DescriptionRabbit polyclonal to KIAA0528
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Sea urchin, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residue 375 and 425 of human KIAA0528 ( NP_055617.1)
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 110 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceKIAA0528 is a protein that was first identified in the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. The function of KIAA0528 has not been characterized. By similarity KO528 contains a C2 domain, a domain that is found in proteins that bind phospholipids. The C2 domain is also present in a family of proteins involved in synaptic vesicle trafficking; thus, the function of KIAA0528 is predicted to be involved in intracellular transport.
- DKFZp779N2044 antibody
- Hypothetical protein LOC9847 antibody
- Uncharacterized protein KIAA0528 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labelling KIAA0528 with ab80470 at 1/400 (2.5µg/ml). Detection: DAB.
All lanes : Anti-KIAA0528 antibody (ab80470) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 110 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Lane 1: ab80470, at 1 µg/mg, staining KIAA0528 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab80470 at 3 µg/mg of lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab80470 has not yet been referenced specifically in any publications.