Key features and details
- Rabbit polyclonal to KIAA0652/ATG13
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KIAA0652/ATG13 antibody
See all KIAA0652/ATG13 primary antibodies
DescriptionRabbit polyclonal to KIAA0652/ATG13
ab105392 is predicted to not cross-react with other ATG members. Two isoforms of KIAA0652/ATG13 are known to exist; this antibody will recognize both isoforms.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human KIAA0652/ATG13 (C terminal).
Database link: NP_001136145
- Rat heart tissue, Mouse heart tissue ICC: DU145 cell line
This product was previously labelled as KIAA0652
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab105392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionAutophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex.
Sequence similaritiesBelongs to the ATG13 metazoan family.
modificationsPhosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin.
Cellular localizationCytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
- Information by UniProt
- ATG13 antibody
- ATG13 autophagy related 13 homolog (S. cerevisiae) antibody
- ATG13_HUMAN antibody
All lanes : Anti-KIAA0652/ATG13 antibody (ab105392) at 1 µg/ml
Lane 1 : Rat heart tissue lysate without blocking peptide
Lane 2 : Rat heart tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 57 kDa
Immunohistochemistry of KIAA0652/ATG13 in Mouse heart, using ab105392 at a dilution of 5 µg/ml.
ICC/IF image of ab105392 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105392, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of ATG13 in Mouse Heart cells using ab105392 at 20 ug/ml.
ab105392 has been referenced in 4 publications.
- Stavoe AK et al. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. Elife 8:N/A (2019). PubMed: 31309927
- Zhou M et al. Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway. Front Pharmacol 10:603 (2019). PubMed: 31214032
- Liu W et al. Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway. Int J Mol Med 37:309-18 (2016). WB . PubMed: 26647915
- Huang J et al. HMGB1 promotes drug resistance in osteosarcoma. Cancer Res 72:230-8 (2012). WB ; Human . PubMed: 22102692