Recombinant
RabMAb

Recombinant Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

Overview

  • Product name

    Anti-KIAA0652/ATG13 antibody [EPR19601]
    See all KIAA0652/ATG13 primary antibodies
  • Description

    Rabbit monoclonal [EPR19601] to KIAA0652/ATG13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KIAA0652/ATG13 aa 150-450. The exact sequence is proprietary.
    Database link: O75143

  • Positive control

    • WB: HeLa, MCF7, A29, A-673, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human cerebellum and hippocampus lysates; mouse and rat brain lysates; HeLa cytoplasmic fraction lysate. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
  • General notes

     

     

     This product was previously labelled as KIAA0652

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IP 1/30.

Target

  • Function

    Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex.
  • Sequence similarities

    Belongs to the ATG13 metazoan family.
  • Post-translational
    modifications

    Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin.
  • Cellular localization

    Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG13 antibody
    • ATG13 autophagy related 13 homolog (S. cerevisiae) antibody
    • ATG13_HUMAN antibody
    • Autophagy related 13 antibody
    • Autophagy related protein 13 antibody
    • Autophagy-related protein 13 antibody
    • FLJ20698 antibody
    • KIAA0652 antibody
    • OTTHUMP00000233321 antibody
    • OTTHUMP00000233322 antibody
    • OTTHUMP00000233323 antibody
    • OTTHUMP00000233324 antibody
    • OTTHUMP00000233325 antibody
    • OTTHUMP00000233326 antibody
    see all

Images

  • All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 20 µg
    Lane 4 : A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-3: 30 seconds; Lane 4: 3 minutes.

    The molecular weight  observed is consistent with what has been described in the literature (PMID:18936157).

  • All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate at 20 µg
    Lane 2 : Human hippocampus lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KIAA0652/ATG13 with ab201467 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • KIAA0652/ATG13 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201467 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10µg (Input).

    Lane 2: ab201467 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201467 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

This product has been referenced in:

  • Tang Y  et al. Overexpression of ribonuclease inhibitor induces autophagy in human colorectal cancer cells via the Akt/mTOR/ULK1 pathway. Mol Med Rep 19:3519-3526 (2019). Read more (PubMed: 30896869) »
  • Yuan J  et al. Autophagy regulates the degeneration of the auditory cortex through the AMPK-mTOR-ULK1 signaling pathway. Int J Mol Med 41:2086-2098 (2018). Read more (PubMed: 29344647) »
See all 3 Publications for this product

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