Overview

  • Product name

    Anti-KIAA1543 antibody [NEZHA-1] - C-terminal
    See all KIAA1543 primary antibodies
  • Description

    Mouse monoclonal [NEZHA-1] to KIAA1543 - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human KIAA1543 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9P1Y5

  • Positive control

    • Whole extract of SW480 cells; MDCK cells;Whole extract of HEK-293T cells overexpressing Human KIAA1543.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.0975% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purification notes

    Purified immunoglobulin.
  • Clonality

    Monoclonal
  • Clone number

    NEZHA-1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use 5-10 µg.

WB Use a concentration of 2.5 - 5 µg/ml. Predicted molecular weight: 135 kDa.
ICC/IF Use a concentration of 2.5 µg/ml.

Target

  • Function

    Microtubule minus-end binding protein that acts as a regulator of microtubule dynamics. Specifically required for zonula adherens biogenesis and maintenance by anchoring microtubules at their minus-ends to zonula adherens, leading to recruit KIFC3 kinesin to junctional site.
  • Sequence similarities

    Belongs to the CAMSAP1 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 CKK domain.
  • Domain

    The CKK domain binds microtubules.
  • Cellular localization

    Cell junction > adherens junction. Cytoplasm. Cytoplasm > cytoskeleton. Scattered in the cytoplasm. Localizes along zonula adherens only at mature cell-cell contacts.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calmodulin regulated spectrin associated protein family, member 3 antibody
    • Calmodulin-regulated spectrin-associated protein 3 antibody
    • CAMP3_HUMAN antibody
    • KIAA1543 antibody
    • NEZHA antibody
    • Protein Nezha antibody
    see all

Images

  • Immunofluorescent analysis of MDCK cells (cold methanol fixed, acetone permeabilized) labeling KIAA1543 with ab214073 at 2.5 μg/mL followed by Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Lane 1 : Anti-KIAA1543 antibody [NEZHA-1] - C-terminal (ab214073) at 2.5 µg/ml
    Lane 2 : Anti-KIAA1543 antibody [NEZHA-1] - C-terminal (ab214073) at 5 µg/ml

    All lanes : Whole extract of SW480 cells

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 135 kDa

  • ab214073 was used to immunoprecipitate KIAA1543 from HEK-293T cells overexpressing Human KIAA1543 whole cell extract. Detection antibody: Rabbit Anti-KIAA1543 (C terminal).

    Lane 1: 5 µg 

    Lane 2: 10 µg 

    Lane 3: Negative control: 10 μg non-relevant antibody used for immunoprecipitation

    Lane 4: Negative control: no cell extract

    Lane 5: Whole extract of HEK-293T cells overexpressing Human KIAA1543

     

References

ab214073 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214073.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up