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antigen sequence, corresponding to amino acids 1877-1978 of Human KIAA1731.
Our Abpromise guarantee covers the use of ab122490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling KIAA1731 with ab12249 shows positivity in the plasma membrane and cytoplasm.
ab122490 at 1/200 dilution staining KIAA1731 in paraffin-embedded human prostate tissue by Immunohistochemistry.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"