Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab193949 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab193949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.
A 1 hour primary incubation is recommended for this product. Preliminary experiments gave an approx 75kDa band in Mouse Adipose tissue and Liver lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 139kDa according to NP_065593.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide, we would welcome your feedback if you have tested this antibody in more suitable positive controls.
FunctionMay play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets.
Sequence similaritiesBelongs to the perilipin family.
Cellular localizationCell membrane. Cytoplasm. Lipid droplet. Nascent lipid droplet surface-associated; association with lipid droplets is triacylglycerol synthesis-dependent.
- Information by UniProt
- Adipocyte protein S3 12 antibody
- Adipocyte protein S3-12 antibody
- hCG1646516 antibody
ab193949 has not yet been referenced specifically in any publications.