Anti-Kidins220 antibody (ab34790)
Key features and details
- Rabbit polyclonal to Kidins220
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-Kidins220 antibody
See all Kidins220 primary antibodies -
Description
Rabbit polyclonal to Kidins220 -
Host species
Rabbit -
Specificity
ab34790 recognises Kidins220 (ARMS). -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide:
ASSESTGFGEERESIL
, corresponding to amino acids 1747-1762 of Human Kidins220 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
-
Function
Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway. -
Tissue specificity
Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines. -
Sequence similarities
Contains 12 ANK repeats.
Contains 1 KAP NTPase domain. -
Domain
The transmembrane domain mediates interaction with NTRK1. -
Post-translational
modificationsTyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF). -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 116478 Rat
- SwissProt: Q9EQG6 Rat
- Unigene: 21470 Rat
-
Alternative names
- Ankyrin repeat-rich membrane-spanning protein antibody
- arms antibody
- KDIS_HUMAN antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Kidins220 antibody (ab34790)ICC/IF image of ab34790 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34790, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
-
Datasheet download
References (7)
ab34790 has been referenced in 7 publications.
- Jaudon F et al. Kidins220/ARMS controls astrocyte calcium signaling and neuron-astrocyte communication. Cell Death Differ 27:1505-1519 (2020). PubMed: 31624352
- Nahorski MS et al. Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development. Sci Rep 8:2340 (2018). PubMed: 29402896
- Eisenhardt AE et al. Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles. Oncotarget 7:26628-52 (2016). PubMed: 27034005
- Cesca F et al. Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels. J Biol Chem 290:18045-55 (2015). PubMed: 26037926
- Marracci S et al. Kidins220/ARMS is dynamically expressed during Xenopus laevis development. Int J Dev Biol 57:787-92 (2013). PubMed: 24307304
- Li J et al. PI3K p110a/Akt signaling negatively regulates secretion of the intestinal peptide neurotensin through interference of granule transport. Mol Endocrinol 26:1380-93 (2012). Human . PubMed: 22700584
- Li J et al. PKD1, PKD2, and Their Substrate Kidins220 Regulate Neurotensin Secretion in the BON Human Endocrine Cell Line. J Biol Chem 283:2614-21 (2008). ICC/IF ; Human . PubMed: 18048355
