Anti-Kidins220 antibody (ab34790)

Submit a review Q&A (1)References (6)

Overview

  • Product name

    Anti-Kidins220 antibody
    See all Kidins220 primary antibodies

  • Description

    Rabbit polyclonal to Kidins220

  • Host species

    Rabbit

  • Specificity

    ab34790 recognises Kidins220 (ARMS).

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide:

    ASSESTGFGEERESIL

    , corresponding to amino acids 1747-1762 of Human Kidins220

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab34790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 220 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.

  • Tissue specificity

    Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.

  • Sequence similarities

    Contains 12 ANK repeats.
    Contains 1 KAP NTPase domain.

  • Domain

    The transmembrane domain mediates interaction with NTRK1.

  • Post-translational
    modifications

    Tyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF).

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ankyrin repeat-rich membrane-spanning protein antibody
      • arms antibody
      • KDIS_HUMAN antibody
      • kidins220 antibody
      • Kinase D interacting substance of 220 kDa antibody
      • Kinase D-interacting substrate of 220 kDa antibody
      • rgd 619949 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Kidins220 antibody (ab34790)
      Immunocytochemistry/ Immunofluorescence - Anti-Kidins220 antibody (ab34790)
      ICC/IF image of ab34790 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34790, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    Publishing research using ab34790? Please let us know so that we can cite the reference in this datasheet.

    ab34790 has been referenced in 6 publications.

    • Nahorski MS  et al. Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development. Sci Rep 8:2340 (2018). PubMed: 29402896
    • Eisenhardt AE  et al. Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles. Oncotarget 7:26628-52 (2016). PubMed: 27034005
    • Cesca F  et al. Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels. J Biol Chem 290:18045-55 (2015). PubMed: 26037926
    • Marracci S  et al. Kidins220/ARMS is dynamically expressed during Xenopus laevis development. Int J Dev Biol 57:787-92 (2013). PubMed: 24307304
    • Li J  et al. PI3K p110a/Akt signaling negatively regulates secretion of the intestinal peptide neurotensin through interference of granule transport. Mol Endocrinol 26:1380-93 (2012). Human . PubMed: 22700584
    • Li J  et al. PKD1, PKD2, and Their Substrate Kidins220 Regulate Neurotensin Secretion in the BON Human Endocrine Cell Line. J Biol Chem 283:2614-21 (2008). ICC/IF ; Human . PubMed: 18048355

    Customer reviews and Q&As

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    Question

    Phone call: Customer looking for antibody against Borrelia burgdorferi.

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    Answer

    Thank you for contacting us. I am sorry we are unable to provide western blot images for anti Borrelia antibodies. I would suggest checking the Swissprot website for checking the molecular weight of unprocessed protein. http://www.uniprot.org/uniprot/?query=Borrelia+burgdorferi&offset=0&sort=score we have 18 anti Borrelia products available on our website. Please have a look and choose as per your requirement. I hope this information is nevertheless helpful. Please do not hesitate to contact us if you need any more advice or information.

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