Key features and details
- Rabbit polyclonal to Kidins220
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Kidins220 antibody
See all Kidins220 primary antibodies
DescriptionRabbit polyclonal to Kidins220
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide, corresponding to a region within amino acids 1706-1771 of Human Kidins220 (NP_065789).
- 293T, H1299, HeLaS3, HepG2, MOLT4, Raji cell lysates and SW480 xenograft.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 197 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionPromotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.
Tissue specificityAbundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.
Sequence similaritiesContains 12 ANK repeats.
Contains 1 KAP NTPase domain.
DomainThe transmembrane domain mediates interaction with NTRK1.
modificationsTyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF).
- Information by UniProt
- Ankyrin repeat-rich membrane-spanning protein antibody
- arms antibody
- KDIS_HUMAN antibody
All lanes : Anti-Kidins220 antibody (ab97345) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 197 kDa
5% SDS PAGE
ab97345 at 1/500 dilution staining Kidins220 in paraffin-embedded SW480 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab97345 has been referenced in 1 publication.
- Wang Y et al. MiR-4638-5p inhibits castration resistance of prostate cancer through repressing Kidins220 expression and PI3K/AKT pathway activity. Oncotarget 7:47444-47464 (2016). PubMed: 27329728