Key features and details
- Rabbit polyclonal to KIF12
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KIF12 antibody
See all KIF12 primary antibodies
DescriptionRabbit polyclonal to KIF12
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human stomach tissue. WB: KIF12 overexpressed HEK-293T cell lysate. ICC/IF: RPTEC TERT1 cells.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254748 in the following tested applications.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed in fetal liver, adult brain and pancreatic islet as well as in kidney tumors, uterus cancer and pancreatic cancer.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Kif12 antibody
- KIF12_HUMAN antibody
- Kinesin family member 12 antibody
Paraffin-embedded human stomach tissue stained for KIF12 using ab254748 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-KIF12 antibody (ab254748) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cells.
Lane 2 : KIF12 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 14 kDa
PFA fixed, Triton X-100 permeabilized RPTEC TERT1 (Human cell line) cells labeling KIF12 using ab254748 at 4µg/ml (green) in ICC/IF.
ab254748 has not yet been referenced specifically in any publications.