Key features and details
- Rabbit polyclonal to KIF13A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KIF13A antibody
DescriptionRabbit polyclonal to KIF13A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residues 1675 and 1725 of human KIF13A (NP_071396.3)
- HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72414 was affinity purified using an epitope specific to KIF13A immobilized on solid support.
Our Abpromise guarantee covers the use of ab72414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 203 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis.
Tissue specificityWidely expressed, with highest levels in heart, brain and skeletal muscle.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Developmental stageExpressed at very low level in most fetal tissues, but at higher levels in skeletal muscle and lung.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Midbody. Endosome membrane. Golgi apparatus membrane. Recruited to the midbody during cytokinesis.
- Information by UniProt
- bA500C11.2 antibody
- FLJ27232 antibody
- Homolog of mouse KIF13A mannose 6 phosphate receptor transporter antibody
All lanes : Anti-KIF13A antibody (ab72414) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 203 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Detection of KIF13A by Western Blot of Immunprecipitate.
ab72414, at 1µg/ml, staining KIF13A in HeLa whole cell lysate immunoprecipitated using ab72414 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab72414 has not yet been referenced specifically in any publications.