Product nameAnti-KIF14 antibody
See all KIF14 primary antibodies
DescriptionRabbit polyclonal to KIF14
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14)encoded within exon 2.
- HeLa whole cell extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab3746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PubMed: 16648480|
|WB||1/2000 - 1/10000. Predicted molecular weight: 190 kDa.|
|IP||Use a concentration of 1 - 4 µg/ml.|
FunctionPlays an essential role in cytokinesis.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Developmental stageUp-regulated in cells progressing through G2/M phase.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
- Information by UniProt
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
Sample: Hela whole cell lysate
1) WB - 50
2) IP - 1mg.
Antibody: Affinity purified Rabbit anti-KIF (ab3746)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3
µg / mg of lysate.
ICC/IF image of ab3746 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Sgrò F et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). Read more (PubMed: 26586574) »
- Carleton M et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF ; Human . Read more (PubMed: 16648480) »