Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF14
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14)encoded within exon 2.

  • Positive control
    • HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 16648480
WB 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
IP Use a concentration of 1 - 4 µg/ml.

Target

  • Function
    Plays an essential role in cytokinesis.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • Developmental stage
    Up-regulated in cells progressing through G2/M phase.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Images

  • Sample: Hela whole cell lysate

    1) WB - 50µg.

    2) IP - 1mg.

     

    Antibody: Affinity purified Rabbit anti-KIF (ab3746)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

     

    Detection: ECL.

     

  • ICC/IF image of ab3746 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Sgrò F  et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). Read more (PubMed: 26586574) »
  • Carleton M  et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF ; Human . Read more (PubMed: 16648480) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwo free of charge replacements with the order numbers ***** for the anti-Vitronectin ab13413 and **** for the anti-Periplakin antibodyab131269.

To check the status of the orders please contact our Customer Service team and reference these numbers.

Please note that these free of charge replacements vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for contacting us and for providing this further information.

As explained over the phone,I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement, a refund or credit note in compensation.
As replacement please find below the list of alternative productswe can send to you. Please note that these 3 antibodies are currently out of stock and should be delivered to you withina week.

anti-KIF14, ab3747, https://www.abcam.com/ab3747
anti-KIF14, ab71155, https://www.abcam.com/ab71155
anti-periplatin, ab131269, https://www.abcam.com/ab131269




I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you for contacting us and for sending an image of your blot.

I am sorry to hear you are experiencing difficulties with two of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am addingsome questions belowso that we can gather further information regarding the samples tested and the protocol used. Once we receive the answers, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

Samples
Species of the sample :
Type of the tissue/cell lysate :

Transfer
Transfer conditions (time and temperature, wet or semi-dry):
Transfer buffer :
Protein transfer verified?

Blocking
Blocking agent and concentration :

Primary antibody
concentration or dilution :
Diluent buffer :
Incubation time :
Incubation temperature :

I look forward to receiving your reply.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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