Overview

  • Product name

  • Description

    Rabbit polyclonal to KIF14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14) encoded within exon 16.

  • Positive control

    • HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/250 - 1/1000.

Target

  • Function

    Plays an essential role in cytokinesis.
  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • Developmental stage

    Up-regulated in cells progressing through G2/M phase.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Images

  • Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KIF14 with ab3747 at 1/1000 (1µg/ml). Detection: DAB.
  • Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lane 1 - Input.

    Lane 2 - IP.

    Lane 3 - Control IgG IP.

References

This product has been referenced in:

  • Schiewek J  et al. Clinical relevance of cytoskeleton associated proteins for ovarian cancer. J Cancer Res Clin Oncol 144:2195-2205 (2018). Read more (PubMed: 30094535) »
See 1 Publication for this product

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