Product nameAnti-KIF14 antibody
See all KIF14 primary antibodies
DescriptionRabbit polyclonal to KIF14
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region between residue 1600 and the C-terminus (residue 1648) of human KIF14 (NP_055690.1).
- Whole cell lysates from Hela cells and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 186 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC||1/200 - 1/1000.
Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
|ICC/IF||Use at an assay dependent concentration.|
FunctionPlays an essential role in cytokinesis.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Developmental stageUp-regulated in cells progressing through G2/M phase.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
- Information by UniProt
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
All lanes : Anti-KIF14 antibody (ab71155) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 186 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Additional bands at: 180 kDa, 190 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling KIF14 with ab71155 at 1/200 (1µg/ml). Detection: DAB.
Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling KIF14 with ab71155 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab71155 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab71155 at 1 µg/ml.