Overview

  • Product name

  • Description

    Rabbit polyclonal to KIF14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1600 and the C-terminus (residue 1648) of human KIF14 (NP_055690.1).

  • Positive control

    • Whole cell lysates from Hela cells and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 186 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/200 - 1/1000.

Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Plays an essential role in cytokinesis.
  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • Developmental stage

    Up-regulated in cells progressing through G2/M phase.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Images

  • All lanes : Anti-KIF14 antibody (ab71155) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Predicted band size: 186 kDa
    Observed band size: 210 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 180 kDa, 190 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling KIF14 with ab71155 at 1/200 (1µg/ml). Detection: DAB.
  • Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling KIF14 with ab71155 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab71155 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab71155 at 1 µg/ml.

References

This product has been referenced in:

  • Huang W  et al. Inhibition of KIF14 Suppresses Tumor Cell Growth and Promotes Apoptosis in Human Glioblastoma. Cell Physiol Biochem 37:1659-70 (2015). Read more (PubMed: 26536004) »
See 1 Publication for this product

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