Key features and details
- Rabbit polyclonal to KIF18A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KIF18A antibody
See all KIF18A primary antibodies
DescriptionRabbit polyclonal to KIF18A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 848 and the C-terminus (residue 898) of human KIF18A (NP_112494.2)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72417 was affinity purified using an epitope specific to KIF18A immobilized on solid support.
Our Abpromise guarantee covers the use of ab72417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMicrotubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Cellular localizationCell projection > ruffle. Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- AU024633 antibody
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All lanes : Anti-KIF18A antibody (ab72417) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Detection of KIF18A by Western Blot of Immunprecipitate.
ab72417 at 1µg/ml staining KIF18A in HeLa whole cell lysate immunoprecipitated using ab72417 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab72417 has not yet been referenced specifically in any publications.