Key features and details
- Rabbit polyclonal to KIF1A
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-KIF1A antibody
See all KIF1A primary antibodies
DescriptionRabbit polyclonal to KIF1A
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Mouse
Predicted to work with: Dog, Human
Synthetic peptide corresponding to Human KIF1A aa 1500-1600 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both Mouse brain embryonic E14 and Mouse brain embryonic E18 tissue lysates. As well as human hippocampus formalin fixed paraffin embedded tissue section within immunohistochemistry.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).|
|ELISA||Use a concentration of 1 µg/ml.|
FunctionMotor for anterograde axonal transport of synaptic vesicle precursors.
Sequence similaritiesBelongs to the kinesin-like protein family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Contains 1 PH domain.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ATSV antibody
- Axonal transporter of synaptic vesicles antibody
- C2orf20 antibody
All lanes : Anti-KIF1A antibody (ab91029) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 191 kDa
Observed band size: 191 kDa
Additional bands at: 58 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
IHC image of KIF1A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91029, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab91029 has been referenced in 1 publication.
- Hung CO & Coleman MP KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons. Traffic 17:1155-1167 (2016). WB ; Mouse . PubMed: 27484852