Recombinant Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Product name

Anti-KIF1A antibody [EPR11790(B)]
See all KIF1A primary antibodies
Description

Rabbit monoclonal [EPR11790(B)] to KIF1A
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide within Human KIF1A aa 900-1000. The exact sequence is proprietary.
Database link: Q12756
Positive control
- HEK-293 cells, Human fetal brain lysates, SH-SY5Y cells, U-87 MG cells, rat hypothalamus lysate and rat brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11790(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HEK293 whole cell lysate (ab7902)

Our Abpromise guarantee covers the use of ab180153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/5000 - 1/10000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
ICC/IF		1/100.
Flow Cyt		1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/50.

Application notes

Is unsuitable for IHC-P.

Function

Motor for anterograde axonal transport of synaptic vesicle precursors.
Sequence similarities

Belongs to the kinesin-like protein family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Contains 1 PH domain.
Cellular localization

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession Q12756 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 547 Human
- Entrez Gene: 363288 Rat
- Omim: 601255 Human
- SwissProt: Q12756 Human
- Unigene: 516802 Human
Alternative names
- ATSV antibody
- Axonal transporter of synaptic vesicles antibody
- C2orf20 antibody
- HSN2C antibody
- hUnc 104 antibody
- hUnc-104 antibody
- hunc104 antibody
- Kif1a antibody
- KIF1A related protein antibody
- KIF1A_HUMAN antibody
- Kinesin family member 1A antibody
- Kinesin heavy chain member 1A homolog of mouse antibody
- kinesin like protein antibody
- kinesin like protein KIF1A antibody
- Kinesin-like protein KIF1A antibody
- Microtubule based motor antibody
- Microtubule based motor KIF1A antibody
- Microtubule-based motor KIF1A antibody
- MRD9 antibody
- SPG30 antibody
- Unc 104 and KIF1A related protein antibody
- Unc 104 antibody
- Unc-104- and KIF1A-related protein antibody
- UNC104 antibody
see all

Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

All lanes : Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/1000 dilution (purified)

Lane 1 : Rat hypothalamus lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 191 kDa
Observed band size: 191 kDa

Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling KIF1A with purified ab180153 at 1/100 dilution (9.2μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the secondary antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as the nuclear counter stain.
Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

ab180153 (purified) at 1/50 dilution (2ug) immunoprecipitating KIF1A in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

Lane 2 (+): ab180153 + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180153 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling KIF1A with purified ab180153 at 1/90 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabelled control, cells without incubation with primary antibody and secondary antibody.
Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (purified) + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 191 kDa
Observed band size: 191 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with unpurified ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/20000 dilution (unpurified) + 293 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 191 kDa
Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Western blot analysis of 293 cell lysate immunoprecipitated using unpurified ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling KIF1A with unpurified ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (unpurified) + Human fetal brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 191 kDa

Flow cytometry protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Pesaresi M et al. Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features. Biol Sex Differ 9:6 (2018). WB ; Rat . Read more (PubMed: 29351809) »
Wang J et al. Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats. Front Cell Neurosci 11:432 (2017). Read more (PubMed: 29375318) »

See all 2 Publications for this product

Publishing research using ab180153? Please let us know so that we can cite the reference in this datasheet.

Recombinant Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Conjugation kits

Isotype control

Positive Controls

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As