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RecombinantRabMAb

Recombinant Anti-KIF1A antibody [EPR11790(B)] (ab180153)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
  • Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11790(B)] to KIF1A
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-KIF1A antibody [EPR11790(B)]
    See all KIF1A primary antibodies
  • Description

    Rabbit monoclonal [EPR11790(B)] to KIF1A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human KIF1A aa 900-1000. The exact sequence is proprietary.
    Database link: Q12756

  • Positive control

    • HEK-293 cells, Human fetal brain lysates, SH-SY5Y cells, U-87 MG cells, rat hypothalamus lysate and rat brain lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11790(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Associated products

  • Alternative Versions

    • Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HEK-293 whole cell lysate (ab7902)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Rat
Human
Application Abreviews Notes
WB
1/5000 - 1/10000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
ICC/IF
1/100.
Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP
1/50.
Notes
WB
1/5000 - 1/10000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
ICC/IF
1/100.
Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP
1/50.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Motor for anterograde axonal transport of synaptic vesicle precursors.
    • Sequence similarities

      Belongs to the kinesin-like protein family. Unc-104 subfamily.
      Contains 1 FHA domain.
      Contains 1 kinesin-motor domain.
      Contains 1 PH domain.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession Q12756 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 547 Human
      • Entrez Gene: 363288 Rat
      • Omim: 601255 Human
      • SwissProt: Q12756 Human
      • Unigene: 516802 Human
      • Alternative names

        • ATSV antibody
        • Axonal transporter of synaptic vesicles antibody
        • C2orf20 antibody
        • HSN2C antibody
        • hUnc 104 antibody
        • hUnc-104 antibody
        • hunc104 antibody
        • Kif1a antibody
        • KIF1A related protein antibody
        • KIF1A_HUMAN antibody
        • Kinesin family member 1A antibody
        • Kinesin heavy chain member 1A homolog of mouse antibody
        • kinesin like protein antibody
        • kinesin like protein KIF1A antibody
        • Kinesin-like protein KIF1A antibody
        • Microtubule based motor antibody
        • Microtubule based motor KIF1A antibody
        • Microtubule-based motor KIF1A antibody
        • MRD9 antibody
        • SPG30 antibody
        • Unc 104 and KIF1A related protein antibody
        • Unc 104 antibody
        • Unc-104- and KIF1A-related protein antibody
        • UNC104 antibody
        see all

      Images

      • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        All lanes : Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/1000 dilution (purified)

        Lane 1 : Rat hypothalamus lysate
        Lane 2 : Rat brain lysate

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 191 kDa
        Observed band size: 191 kDa



        Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      • Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

        Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling KIF1A with purified ab180153 at 1/100 dilution (9.2μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the secondary antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as the nuclear counter stain.

      • Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

        ab180153 (purified) at 1/50 dilution (2ug) immunoprecipitating KIF1A in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

        Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

        Lane 2 (+): ab180153 + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180153 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

        For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000). 

        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

      • Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

        Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling KIF1A with purified ab180153 at 1/90 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabelled control, cells without incubation with primary antibody and secondary antibody.

      • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (purified) + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 191 kDa
        Observed band size: 191 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      • Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with unpurified ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.

      • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/20000 dilution (unpurified) + 293 cell lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 191 kDa

      • Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

        Western blot analysis of 293 cell lysate immunoprecipitated using unpurified ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

      • Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

        Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling KIF1A with unpurified ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

      • Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (unpurified) + Human fetal brain lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 191 kDa

      • Anti-KIF1A antibody [EPR11790(B)] (ab180153)
        Anti-KIF1A antibody [EPR11790(B)] (ab180153)

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (3)

      Publishing research using ab180153? Please let us know so that we can cite the reference in this datasheet.

      ab180153 has been referenced in 3 publications.

      • Pesaresi M  et al. Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features. Biol Sex Differ 9:6 (2018). WB ; Rat . PubMed: 29351809
      • Wang J  et al. Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats. Front Cell Neurosci 11:432 (2017). PubMed: 29375318
      • Du W  et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab180153.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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