Anti-KIF1C antibody (ab72238)
Key features and details
- Rabbit polyclonal to KIF1C
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF1C antibody
See all KIF1C primary antibodies -
Description
Rabbit polyclonal to KIF1C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human KIF1C aa 850-950.
Database link: O43896 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Motor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. Has a microtubule plus end-directed motility. -
Tissue specificity
Expressed in all tissues examined, with most abundant expression in heart and skeletal muscle. -
Involvement in disease
Spastic ataxia 2, autosomal recessive -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin motor domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 10749 Human
- Omim: 603060 Human
- SwissProt: O43896 Human
- Unigene: 435120 Human
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Alternative names
- Kif1c antibody
- KIF1C_HUMAN antibody
- Kinesin family member 1C antibody
see all
Images
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All lanes : Anti-KIF1C antibody (ab72238) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 123 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Various other bands are detected from 31 kDa upwards. -
Immunoprecipitation/ Western Blot of KIF1C.
Lane 1: ab72238 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72238 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72238 has been referenced in 2 publications.
- Gifford V et al. Coordination of two kinesin superfamily motor proteins, KIF3A and KIF13A, is essential for pericellular matrix degradation by membrane-type 1 matrix metalloproteinase (MT1-MMP) in cancer cells. Matrix Biol 107:1-23 (2022). PubMed: 35122963
- Jones LA et al. Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion. J Cell Biol 207:499-516 (2014). PubMed: 25422374