Product nameAnti-KIF20A antibody
See all KIF20A primary antibodies
DescriptionRabbit polyclonal to KIF20A
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human KIF20A aa 50-100. The exact sequence is proprietary. NP_005724.1
Database link: O95235
- IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264155 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
modificationsPhosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
Cellular localizationGolgi apparatus. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
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KIF20A was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264155 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different KIF20A antibody at 1 µg/ml.
Lane 1: ab264155 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
ab264155 has not yet been referenced specifically in any publications.