Anti-KIF21B antibody (ab121931)

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Key features and details

  • Rabbit polyclonal to KIF21B
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-KIF21B antibody

  • Description

    Rabbit polyclonal to KIF21B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen: RKTQEVSALR RLAKPMSERV AGRAGLKPPM LDSGAEVSAS TTSSEAESGA RSVSSIVRQW NRKINHFLGD HPAPTVNGTR PARKKFQKKG, corresponding to amino acids 814-903 of Human KIF21B.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human kidney tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Microtubule-binding motor protein probably involved in neuronal dendritic transport. In vitro, has a plus-end directed motor activity.

  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
    Contains 7 WD repeats.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • KI21B_HUMAN antibody
      • KIAA0449 antibody
      • KIF21B antibody
      • Kinesin-like protein KIF21B antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KIF21B antibody (ab121931)
      Immunocytochemistry/ Immunofluorescence - Anti-KIF21B antibody (ab121931)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121931 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody (ab121931)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody (ab121931)
      ab121931 at 1/125 dilution, staining KIF21B in paraffin embedded Human kidney tissue by Immunohistochemistry.

    References (1)

    Publishing research using ab121931? Please let us know so that we can cite the reference in this datasheet.

    ab121931 has been referenced in 1 publication.

    • Sun ZG  et al. Kinesin superfamily protein 21B acts as an oncogene in non-small cell lung cancer. Cancer Cell Int 20:233 (2020). PubMed: 32536821

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