Anti-KIF21B antibody (ab121931)
Key features and details
- Rabbit polyclonal to KIF21B
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF21B antibody -
Description
Rabbit polyclonal to KIF21B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: RKTQEVSALR RLAKPMSERV AGRAGLKPPM LDSGAEVSAS TTSSEAESGA RSVSSIVRQW NRKINHFLGD HPAPTVNGTR PARKKFQKKG, corresponding to amino acids 814-903 of Human KIF21B.
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Positive control
- Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Microtubule-binding motor protein probably involved in neuronal dendritic transport. In vitro, has a plus-end directed motor activity. -
Sequence similarities
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 23046 Human
- Omim: 608322 Human
- SwissProt: O75037 Human
- Unigene: 169182 Human
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Alternative names
- KI21B_HUMAN antibody
- KIAA0449 antibody
- KIF21B antibody
- Kinesin-like protein KIF21B antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KIF21B antibody (ab121931)Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121931 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody (ab121931)ab121931 at 1/125 dilution, staining KIF21B in paraffin embedded Human kidney tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab121931 has been referenced in 1 publication.
- Sun ZG et al. Kinesin superfamily protein 21B acts as an oncogene in non-small cell lung cancer. Cancer Cell Int 20:233 (2020). PubMed: 32536821
