Overview

  • Product name
    Anti-KIF21B antibody
    See all KIF21B primary antibodies
  • Description
    Rabbit polyclonal to KIF21B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: RKTQEVSALR RLAKPMSERV AGRAGLKPPM LDSGAEVSAS TTSSEAESGA RSVSSIVRQW NRKINHFLGD HPAPTVNGTR PARKKFQKKG, corresponding to amino acids 814-903 of Human KIF21B.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Microtubule-binding motor protein probably involved in neuronal dendritic transport. In vitro, has a plus-end directed motor activity.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
    Contains 7 WD repeats.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
    • Alternative names
      • KI21B_HUMAN antibody
      • KIAA0449 antibody
      • KIF21B antibody
      • Kinesin-like protein KIF21B antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KIF21B antibody (ab121931)
      Immunocytochemistry/ Immunofluorescence - Anti-KIF21B antibody (ab121931)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121931 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody (ab121931)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody (ab121931)
      ab121931 at 1/125 dilution, staining KIF21B in paraffin embedded Human kidney tissue by Immunohistochemistry.

    References

    ab121931 has not yet been referenced specifically in any publications.

    Publishing research using ab121931? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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