Overview

  • Product name

    Anti-KIF22 antibody [EP2747Y]
    See all KIF22 primary antibodies
  • Description

    Rabbit monoclonal [EP2747Y] to KIF22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KIF22 (N terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2747Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP 1/10.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • A 328A3.2 antibody
    • KID antibody
    • KIF 22 antibody
    • kif22 antibody
    • KIF22_HUMAN antibody
    • Kinesin family member 22 antibody
    • Kinesin like 4 antibody
    • Kinesin like DNA binding protein antibody
    • Kinesin like DNA binding protein pseudogene antibody
    • Kinesin like protein 4 antibody
    • Kinesin like protein KIF22 antibody
    • Kinesin-like DNA-binding protein antibody
    • Kinesin-like protein 4 antibody
    • Kinesin-like protein KIF22 antibody
    • KNSL 4 antibody
    • KNSL4 antibody
    • OBP 1 antibody
    • OBP 2 antibody
    • OBP antibody
    • OBP1 antibody
    • OBP2 antibody
    • Origin of plasmid DNA replication binding protein antibody
    • OriP binding protein antibody
    • OTTHUMP00000123406 antibody
    • SEMDJL2 antibody
    see all

Images

  • Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution + HeLa cell
    lysate at 10 µg

    Secondary
    HRP labelled oat anti rabbit at 1/2000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

References

This product has been referenced in:

  • Li C  et al. NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nat Commun 7:10597 (2016). IF . Read more (PubMed: 26839278) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - nuclear (cartilage)
Loading amount
10 µg
Specification
cartilage
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 26 2012

Answer

Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. I have checked the alignment of the immunogen sequences for the available antibodies with the zebrafish sequence. I am sorry that the alignment is very low in all cases, and regrettably we would not predict that any of the KIF22 antibodies would detect in Zebrafish. I am sorry that this is not better news for you on this occasion, and that we have no suitably tested KIFF22 antibodies available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions.          

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