Overview

  • Product name

    Anti-KIF22 antibody [EP2748]
    See all KIF22 primary antibodies
  • Description

    Rabbit monoclonal [EP2748] to KIF22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KIF22 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • HeLa, MCF7, and SH-SY5Y cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2748
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
    • Sequence similarities

      Belongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • Post-translational
      modifications

      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • A 328A3.2 antibody
      • KID antibody
      • KIF 22 antibody
      • kif22 antibody
      • KIF22_HUMAN antibody
      • Kinesin family member 22 antibody
      • Kinesin like 4 antibody
      • Kinesin like DNA binding protein antibody
      • Kinesin like DNA binding protein pseudogene antibody
      • Kinesin like protein 4 antibody
      • Kinesin like protein KIF22 antibody
      • Kinesin-like DNA-binding protein antibody
      • Kinesin-like protein 4 antibody
      • Kinesin-like protein KIF22 antibody
      • KNSL 4 antibody
      • KNSL4 antibody
      • OBP 1 antibody
      • OBP 2 antibody
      • OBP antibody
      • OBP1 antibody
      • OBP2 antibody
      • Origin of plasmid DNA replication binding protein antibody
      • OriP binding protein antibody
      • OTTHUMP00000123406 antibody
      • SEMDJL2 antibody
      see all

    Images

    • All lanes : Anti-KIF22 antibody [EP2748] (ab108535) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 73 kDa

    References

    ab108535 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. I have checked the alignment of the immunogen sequences for the available antibodies with the zebrafish sequence. I am sorry that the alignment is very low in all cases, and regrettably we would not predict that any of the KIF22 antibodies would detect in Zebrafish. I am sorry that this is not better news for you on this occasion, and that we have no suitably tested KIFF22 antibodies available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions.          

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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