Product nameAnti-KIF24 antibody - N-terminal
See all KIF24 primary antibodies
DescriptionRabbit polyclonal to KIF24 - N-terminal
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human KIF24 aa 363-392 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q5T7B8
- Mouse heart tissue and lysate, CEM cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab175527 is purified through a protein A column, followed by immunogen affinity purification.
Our Abpromise guarantee covers the use of ab175527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 152 kDa.|
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- bA571F15.4 antibody
- C9orf48 antibody
- Kif24 antibody
Flow cytometric analysis of CEM cells labeling KIF24 with ab175527 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed, paraffin embedded Mouse heart tissue labeling KIF24 with ab175527 at 1/150 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-KIF24 antibody - N-terminal (ab175527) at 1/1000 dilution + Mouse heart tissue lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
ab175527 has not yet been referenced specifically in any publications.