Anti-KIF24 antibody - N-terminal (ab175527)

Overview

  • Product name
    Anti-KIF24 antibody - N-terminal
    See all KIF24 primary antibodies
  • Description
    Rabbit polyclonal to KIF24 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human KIF24 aa 363-392 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5T7B8

  • Positive control
    • Mouse heart tissue and lysate, CEM cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175527 is purified through a protein A column, followed by immunogen affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab175527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 152 kDa.

Target

Images

  • Flow cytometric analysis of CEM cells labeling KIF24 with ab175527 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Mouse heart tissue labeling KIF24 with ab175527 at 1/150 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-KIF24 antibody - N-terminal (ab175527) at 1/1000 dilution + Mouse heart tissue lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 152 kDa

References

ab175527 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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