• Product name
  • Description
    Rabbit polyclonal to KIF3A
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.

  • Positive control
    • WB: HeLa and MDBK cell lysate. ICC/IF: Primary hippocampal neurons. IHC-P: Rat brain tiisue; mouse brain tissue.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.



Our Abpromise guarantee covers the use of ab11259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 85 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100.


  • Function
    Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIF 3 antibody
    • KIF 3A antibody
    • KIF3 antibody
    • Kif3a antibody
    • KIF3A_HUMAN antibody
    • Kifl antibody
    • Kinesin family member 3A antibody
    • Kinesin family protein 3 antibody
    • Kinesin family protein 3A antibody
    • Kinesin like protein KIF 3A antibody
    • Kinesin like protein KIF3A antibody
    • Kinesin-like protein KIF3A antibody
    • Kns3 antibody
    • Microtubule plus end directed kinesin motor 3A antibody
    • Microtubule plus end-directed kinesin motor 3A antibody
    see all


  • ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.

    See Abreview

  • Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.

  • Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.

  • ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview


This product has been referenced in:
  • Yasuda K  et al. FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination. J Cell Biol 216:1015-1034 (2017). Read more (PubMed: 28298410) »
  • Hoang-Minh LB  et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol 9:392-402 (2016). WB ; Human . Read more (PubMed: 27661404) »
See all 19 Publications for this product

Customer reviews and Q&As

11-12 of 12 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (HeLa)
Loading amount
200000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 13 2008


Thank you for your enquiry. ab11259 was verified to work by immunohistochemistry using frozen sections of rat cerebellum at a 1:200 dilution. You may find it useful to refer to the paper below that characterises its application. Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport Nobutaka Hirokawa Science 23 January 1998; 279: 519-526. Please do not hesitate to contact me should you require further assistance.

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11-12 of 12 Abreviews or Q&A

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